Strain Identification of Mycobacterium Tuberculosis by DNA Fingerprinting: Recommendations for a Standardized Methodology

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1993 Feb 1

PMID 8381814

Citations 963

Authors

J D van Embden

M D Cave

J T Crawford

J W Dale

K D Eisenach

B Gicquel

P Hermans

C Martin

R McAdam

T M Shinnick

Affiliations

Soon will be listed here.

Abstract

DNA fingerprinting of Mycobacterium tuberculosis has been shown to be a powerful epidemiologic tool. We propose a standardized technique which exploits variability in both the number and genomic position of IS6110 to generate strain-specific patterns. General use of this technique will permit comparison of results between different laboratories. Such comparisons will facilitate investigations into the international transmission of tuberculosis and may identify specific strains with unique properties such as high infectivity, virulence, or drug resistance.

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References

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