J W Dale
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Explore the profile of J W Dale including associated specialties, affiliations and a list of published articles.
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86
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Recent Articles
1.
Mardare C, Delahay R, Dale J
J Appl Microbiol
. 2013 Feb;
114(5):1388-94.
PMID: 23398391
Aims: To test the hypothesis that Mycobacterium bovis can persist in the environment within protozoa. Methods And Results: In this study, we used a novel approach to detect internalized mycobacteria...
2.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:293-300.
PMID: 21374209
After ligation and transformation, a number of clones will (it is hoped) be obtained that can be identified as recombinants by the occurrence of insertional inactivation, by analysis of small-scale...
3.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:289-92.
PMID: 21374208
There are a number of methods available for screening either potential recombinant clones or natural isolates for the possession of plasmids. The method described in this chapter is based on...
4.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:285-8.
PMID: 21374207
Phage vectors are often used rather than plasmids, particularly for the production of gene "banks" or "libraries." The plaques produced can be screened for the presence of specific DNA sequences...
5.
Dale J, J P
Methods Mol Biol
. 2011 Mar;
2:277-83.
PMID: 21374206
After transformation, cells containing parental or recombinant plasmids are usually selected by taking advantage of an antibiotic resistance marker present on the vector plasmid. Cells containing recombinant plasmids can often...
6.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:245-50.
PMID: 21374200
In the normal growth cycle of bacteriophage lambda, the proteins that ultimately form the head of the phage particle are assembled into an empty precursor of the head (prehead); the...
7.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:241-4.
PMID: 21374199
The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a...
8.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:231-6.
PMID: 21374197
Linearized plasmid vector molecules produced by restriction enzyme digestion can be reformed into circles by the action of DNA ligase. This is the most favorable reaction even when foreign DNA...
9.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:211-5.
PMID: 21374194
The isolation of phage DNA from a purified phage preparation simply involves the removal of the proteins of the phage particle. This is done most easily by phenol extraction (Method...
10.
Dale J, Greenaway P
Methods Mol Biol
. 2011 Mar;
2:201-9.
PMID: 21374193
Lambda, a temperate bacteriophage of E. coli, has two alternative modes of replication in sensitive cells, known as the lytic and lysogenic cycles. In the lytic cycle, after the lambda...