Molecular Epidemiology of Clinical Isolates from Southern Xinjiang, China Using Spoligotyping and 15-Locus MIRU-VNTR Typing

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2023 Mar 15

PMID 36919034

Authors

Chunjie Yin

Xiaokaiti Mijiti

Haican Liu

Quan Wang

Bin Cao

Aiketaguli Anwaierjiang

Machao Li

Mengwen Liu

Yi Jiang

Miao Xu

Kanglin Wan

Xiuqin Zhao

Guilian Li

Hui Xiao

Affiliations

Soon will be listed here.

Abstract

Background: In the last decades, the molecular epidemiological investigation of has significantly increased our understanding of tuberculosis epidemiology. However, few such studies have been done in southern Xinjiang, China. We aimed to clarify the molecular epidemic characteristics and their association with drug resistance in the isolates circulating in this area.

Methods: A total of 347 isolates obtained from southern Xinjiang, China between Sep, 2017 and Sep, 2019 were included to characterize using a 15-locus MIRU-VNTR (VNTR-15) typing and spoligotyping, and test for drug susceptibility profiles. Then the lineages and clustering of the isolates were analyzed, as well as their association with drug resistance.

Results: Spoligotyping results showed that 60 spoligotype international types (SITs) containing 35 predefined SITs and 25 Orphan or New patterns, and 12 definite genotypes were found, and the top three prevalent genotypes were Beijing genotype (207, 59.7%), followed by CAS1-Delhi (46, 13.6%), and Ural-2 (30, 8.6%). The prevalence of Beijing genotype infection in the younger age group (≤30) was more frequent than the two older groups (30~59 and ≥60 years old, both values <0.05). The Beijing genotype showed significantly higher prevalence of resistance to isoniazid, rifampicin, ethambutol, multi-drug or at least one drug than the non-Beijing genotype (All values ≤0.05). The estimated proportion of tuberculosis cases due to transmission was 18.4% according to the cluster rate acquired by VNTR-15 typing, and the Beijing genotype was the risk factor for the clustering (OR 9.15, 95% CI: 4.18-20.05).

Conclusion: Our data demonstrated that the Beijing genotype is the dominant lineage, associated with drug resistance, and was more likely to infect young people and contributed to tuberculosis transmission in southern Xinjiang, China. These findings will contribute to a better understanding of tuberculosis epidemiology in this area.

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