Use of Gene Probes Based on the Insertion Sequence IS986 to Differentiate Between BCG Vaccine Strains

Overview

Journal J Appl Bacteriol

Specialty Microbiology

Date 1992 Feb 1

PMID 1313411

Citations 24

Authors

N G Fomukong

J W Dale

T W Osborn

J M Grange

Affiliations

Soon will be listed here.

Abstract

Gene probes derived from the insertion sequence IS986, which have previously been shown to differentiate isolates of Mycobacterium tuberculosis for epidemiological analysis, are also capable of distinguishing two groups of BCG vaccine strains. Most BCG strains have a single copy of IS986, at the same chromosomal site, while the Brazilian, Japanese and USSR strains have an additional copy at a different, common location. These results correlate with the results of previous antigenic analysis and may reflect a different clonal origin of the two groups of BCG strains.

Citing Articles

A Two-Step Real-Time PCR Method To Identify Mycobacterium tuberculosis Infections and Six Dominant Nontuberculous Mycobacterial Infections from Clinical Specimens.

Park J, Kwak N, Chae J, Yoon E, Jeong S Microbiol Spectr. 2023; 11(4):e0160623.

PMID: 37378523 PMC: 10434164. DOI: 10.1128/spectrum.01606-23.

Vaccines Against Tuberculosis: Problems and Prospects (Review).

Nadolinskaia N, Karpov D, Goncharenko A Appl Biochem Microbiol. 2020; 56(5):497-504.

PMID: 32981943 PMC: 7508421. DOI: 10.1134/S0003683820050129.

Complete Genome Sequence of Mycobacterium bovis Strain BCG-1 (Russia).

Sotnikova E, Shitikov E, Malakhova M, Kostryukova E, Ilina E, Atrasheuskaya A Genome Announc. 2016; 4(2).

PMID: 27034492 PMC: 4816620. DOI: 10.1128/genomeA.00182-16.

Evolution of M. bovis BCG Vaccine: Is Niacin Production Still a Valid Biomarker?.

Singh S, Kumar M, Singh P Tuberc Res Treat. 2015; 2015:957519.

PMID: 25694828 PMC: 4324913. DOI: 10.1155/2015/957519.

Mapping IS6110 in high-copy number Mycobacterium tuberculosis strains shows specific insertion points in the Beijing genotype.

Alonso H, Samper S, Martin C, Otal I BMC Genomics. 2013; 14:422.

PMID: 23800083 PMC: 3701491. DOI: 10.1186/1471-2164-14-422.