A Mutant Allele of the SUP45 (SAL4) Gene of Saccharomyces Cerevisiae Shows Temperature-dependent Allosuppressor and Omnipotent Suppressor Phenotypes

Overview

Journal Curr Genet

Specialty Genetics

Date 1995 Apr 1

PMID 7586027

Citations 38

Authors

I Stansfield

Akhmaloka

M F Tuite

Affiliations

Soon will be listed here.

Abstract

Using a plasmid-based termination-read-through assay, the sal4-2 conditional-lethal (temperature-sensitive) allele of the SUP45 (SAL4) gene was shown to enhance the efficiency of the weak ochre suppressor tRNA SUQ5 some 10-fold at 30 degrees C. Additionally, this allele increased the suppressor efficiency of SRM2-2, a weak tRNA(Gln) ochre suppressor, indicating that the allosuppressor phenotype is not SUQ5-specific. A sup+ sal4-2 strain also showed a temperature-dependent omnipotent suppressor phenotype, enhancing readthrough of all three termination codons. Combining the sal4-2 allele with an efficient tRNA nonsense suppressor (SUP4) increased the temperature-sensitivity of that strain, indicating that enhanced nonsense suppressor levels contribute to the conditional-lethality conferred by the sal4-2 allele. However, UGA suppression levels in a sup+ sal4-2 strain following a shift to the non-permissive temperature reached a maximum significantly below that exhibited by a non-temperature sensitive SUP4 suppressor strain. Enhanced nonsense suppression may not therefore be the primary cause of the conditional-lethality of this allele. These data indicate a role for Sup45p in translation termination, and possibly in an additional, as yet unidentified, cellular process.

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References

Crouzet M, Izgu F, Grant C, Tuite M . The allosuppressor gene SAL4 encodes a protein important for maintaining translational fidelity in Saccharomyces cerevisiae. Curr Genet. 1988; 14(6):537-43. DOI: 10.1007/BF00434078. View

Finkelstein D, Strausberg S . Heat shock-regulated production of Escherichia coli beta-galactosidase in Saccharomyces cerevisiae. Mol Cell Biol. 1983; 3(9):1625-33. PMC: 370016. DOI: 10.1128/mcb.3.9.1625-1633.1983. View

Rosset R, GORINI L . A ribosomal ambiguity mutation. J Mol Biol. 1969; 39(1):95-112. DOI: 10.1016/0022-2836(69)90336-2. View

Leeds P, Wood J, Lee B, Culbertson M . Gene products that promote mRNA turnover in Saccharomyces cerevisiae. Mol Cell Biol. 1992; 12(5):2165-77. PMC: 364388. DOI: 10.1128/mcb.12.5.2165-2177.1992. View

Laten H, Gorman J, Bock R . Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae. Nucleic Acids Res. 1978; 5(11):4329-42. PMC: 342752. DOI: 10.1093/nar/5.11.4329. View

Manivasakam P, Schiestl R . High efficiency transformation of Saccharomyces cerevisiae by electroporation. Nucleic Acids Res. 1993; 21(18):4414-5. PMC: 310097. DOI: 10.1093/nar/21.18.4414. View

Sherman F . Getting started with yeast. Methods Enzymol. 1991; 194:3-21. DOI: 10.1016/0076-6879(91)94004-v. View

Brown C, Stockwell P, Trotman C, Tate W . Sequence analysis suggests that tetra-nucleotides signal the termination of protein synthesis in eukaryotes. Nucleic Acids Res. 1990; 18(21):6339-45. PMC: 332501. DOI: 10.1093/nar/18.21.6339. View

Hoffman C, Winston F . A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene. 1987; 57(2-3):267-72. DOI: 10.1016/0378-1119(87)90131-4. View

10.

Rose M, Novick P, Thomas J, Botstein D, Fink G . A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene. 1987; 60(2-3):237-43. DOI: 10.1016/0378-1119(87)90232-0. View

11.

Vincent A, Liebman S . The yeast omnipotent suppressor SUP46 encodes a ribosomal protein which is a functional and structural homolog of the Escherichia coli S4 ram protein. Genetics. 1992; 132(2):375-86. PMC: 1205143. DOI: 10.1093/genetics/132.2.375. View

12.

Mindich L . A mutation that increases the activity of nonsense suppressors in Escherichia coli. Mol Gen Genet. 1978; 159(2):161-9. DOI: 10.1007/BF00270889. View

13.

Liebman S, Sherman F . Inhibition of growth by amber suppressors in yeast. Genetics. 1976; 82(2):233-49. PMC: 1213454. DOI: 10.1093/genetics/82.2.233. View

14.

All-Robyn J, Brown N, Otaka E, Liebman S . Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein. Mol Cell Biol. 1990; 10(12):6544-53. PMC: 362931. DOI: 10.1128/mcb.10.12.6544-6553.1990. View

15.

Boone C, Clark K, Sprague Jr G . Identification of a tRNA(Gln) ochre suppressor in Saccharomyces cerevisiae. Nucleic Acids Res. 1992; 20(17):4661. PMC: 334201. DOI: 10.1093/nar/20.17.4661. View

16.

Szostak J . Yeast recombination: the association between double-strand gap repair and crossing-over. Proc Natl Acad Sci U S A. 1983; 80(14):4417-21. PMC: 384049. DOI: 10.1073/pnas.80.14.4417. View

17.

Konecki D, Aune K, Tate W, Caskey C . Characterization of reticulocyte release factor. J Biol Chem. 1977; 252(13):4514-20. View

18.

Didichenko S, Ter-Avanesyan M, Smirnov V . Ribosome-bound EF-1 alpha-like protein of yeast Saccharomyces cerevisiae. Eur J Biochem. 1991; 198(3):705-11. DOI: 10.1111/j.1432-1033.1991.tb16070.x. View

19.

Goodman H, Olson M, Hall B . Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A. 1977; 74(12):5453-7. PMC: 431761. DOI: 10.1073/pnas.74.12.5453. View

20.

Eustice D, Wakem L, Wilhelm J, Sherman F . Altered 40 S ribosomal subunits in omnipotent suppressors of yeast. J Mol Biol. 1986; 188(2):207-14. DOI: 10.1016/0022-2836(86)90305-0. View