The Allosuppressor Gene SAL4 Encodes a Protein Important for Maintaining Translational Fidelity in Saccharomyces Cerevisiae

Overview

Journal Curr Genet

Specialty Genetics

Date 1988 Dec 1

PMID 3072098

Citations 16

Authors

M Crouzet

F Izgu

C M Grant

M F Tuite

Affiliations

Soon will be listed here.

Abstract

Allosuppressor (sal) mutations enhance the efficiency of the yeast ochre suppressor SUQ5 and define five unlinked loci, SAL1-SAL5. A number of sal4 mutants were isolated and found to have pleiotropic, allele;specific phenotypes, including hypersensitivity in vivo to paromomycin and other antibiotics that stimulate translational errors in yeast. To examine further the nature of the SAL4 gene product, the wild type SAL4 gene was isolated by complementation of a conditional lethal allele sal4-2, and demonstrated to be a single copy gene encoding a single 1.6 kb transcript. Restriction mapping and DNA hybridisation analysis were used to demonstrate that the SAL4 gene is identical to the previously identified omnipotent suppressor gene SUP45 (SUP1). Our results implicate the SAL4 gene product as playing a major role in maintaining translational accuracy in yeast.

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References

Himmelfarb H, Maicas E, Friesen J . Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product. Mol Cell Biol. 1985; 5(4):816-22. PMC: 366786. DOI: 10.1128/mcb.5.4.816-822.1985. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

McMaster G, Carmichael G . Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977; 74(11):4835-8. PMC: 432050. DOI: 10.1073/pnas.74.11.4835. View

Weiss R, Murphy J, Gallant J . Genetic screen for cloned release factor genes. J Bacteriol. 1984; 158(1):362-4. PMC: 215426. DOI: 10.1128/jb.158.1.362-364.1984. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Thompson R, Dix D, Karim A . The reaction of ribosomes with elongation factor Tu.GTP complexes. Aminoacyl-tRNA-independent reactions in the elongation cycle determine the accuracy of protein synthesis. J Biol Chem. 1986; 261(11):4868-74. View

Harley C, Pollard J, Stanners C, Goldstein S . Model for messenger RNA translation during amino acid starvation applied to the calculation of protein synthetic error rates. J Biol Chem. 1981; 256(21):10786-94. View

Tuite M, McLaughlin C . The effects of paromomycin on the fidelity of translation in a yeast cell-free system. Biochim Biophys Acta. 1984; 783(2):166-70. DOI: 10.1016/0167-4781(84)90009-5. View

Coppin-Raynal E, Picard-Bennoun M, Madjar J . At least seven ribosomal proteins are involved in the control of translational accuracy in a eukaryotic organism. J Mol Biol. 1986; 190(2):167-75. DOI: 10.1016/0022-2836(86)90290-1. View

10.

Eustice D, Wakem L, Wilhelm J, Sherman F . Altered 40 S ribosomal subunits in omnipotent suppressors of yeast. J Mol Biol. 1986; 188(2):207-14. DOI: 10.1016/0022-2836(86)90305-0. View

11.

Holmes D, Quigley M . A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981; 114(1):193-7. DOI: 10.1016/0003-2697(81)90473-5. View

12.

Kushnirov V, Ter-Avanesyan M, Telckov M, Surguchov A, Smirnov V, Inge-Vechtomov S . Nucleotide sequence of the SUP2 (SUP35) gene of Saccharomyces cerevisiae. Gene. 1988; 66(1):45-54. DOI: 10.1016/0378-1119(88)90223-5. View

13.

Cryer D, Eccleshall R, MARMUR J . Isolation of yeast DNA. Methods Cell Biol. 1975; 12:39-44. DOI: 10.1016/s0091-679x(08)60950-4. View

14.

Song J, Liebman S . Allosuppressors that enhance the efficiency of omnipotent suppressors in Saccharomyces cerevisiae. Genetics. 1987; 115(3):451-60. PMC: 1216348. DOI: 10.1093/genetics/115.3.451. View

15.

McIntosh E, HAYNES R . Sequence and expression of the dCMP deaminase gene (DCD1) of Saccharomyces cerevisiae. Mol Cell Biol. 1986; 6(5):1711-21. PMC: 367699. DOI: 10.1128/mcb.6.5.1711-1721.1986. View

16.

Breining P, Piepersberg W . Yeast omnipotent supressor SUP1 (SUP45): nucleotide sequence of the wildtype and a mutant gene. Nucleic Acids Res. 1986; 14(13):5187-97. PMC: 311534. DOI: 10.1093/nar/14.13.5187. View

17.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

18.

Tapio S, Kurland C . Mutant EF-Tu increases missense error in vitro. Mol Gen Genet. 1986; 205(1):186-8. DOI: 10.1007/BF02428051. View

19.

Liebman S, Cavenagh M . An antisuppressor that acts on omnipotent suppressors in yeast. Genetics. 1980; 95(1):49-61. PMC: 1214221. DOI: 10.1093/genetics/95.1.49. View

20.

Wilson P, Culbertson M . SUF12 suppressor protein of yeast. A fusion protein related to the EF-1 family of elongation factors. J Mol Biol. 1988; 199(4):559-73. DOI: 10.1016/0022-2836(88)90301-4. View