Sensitivity of Yeast to Lithium Chloride Connects the Activity of YTA6 and YPR096C to Translation of Structured MRNAs

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Jul 9

PMID 32639961

Citations 6

Authors

Maryam Hajikarimlou

Houman Moteshareie

Katayoun Omidi

Mohsen Hooshyar

Sarah Shaikho

Tom Kazmirchuk

Daniel Burnside

Sarah Takallou

Narges Zare

Sasi Kumar Jagadeesan

Nathalie Puchacz

Mohan Babu

Myron Smith

Martin Holcik

Bahram Samanfar

Ashkan Golshani

Affiliations

Soon will be listed here.

Abstract

Lithium Chloride (LiCl) toxicity, mode of action and cellular responses have been the subject of active investigations over the past decades. In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5'-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA.

Citing Articles

Investigating the Activities of and in the Regulation of mRNA Translation.

Al-Gafari M, Jagadeesan S, Kazmirchuk T, Takallou S, Wang J, Hajikarimlou M Biology (Basel). 2024; 13(11).

PMID: 39596839 PMC: 11592143. DOI: 10.3390/biology13110884.

Biological factors and statistical limitations prevent detection of most noncanonical proteins by mass spectrometry.

Wacholder A, Carvunis A PLoS Biol. 2023; 21(12):e3002409.

PMID: 38048358 PMC: 10721188. DOI: 10.1371/journal.pbio.3002409.

A vast evolutionarily transient translatome contributes to phenotype and fitness.

Wacholder A, Parikh S, Coelho N, Acar O, Houghton C, Chou L Cell Syst. 2023; 14(5):363-381.e8.

PMID: 37164009 PMC: 10348077. DOI: 10.1016/j.cels.2023.04.002.

and Are Involved in Cell Sensitivity to LiCl by Regulating the Translation of mRNA.

Jagadeesan S, Al-Gafari M, Wang J, Takallou S, Allard D, Hajikarimlou M Int J Mol Sci. 2023; 24(2).

PMID: 36675300 PMC: 9864399. DOI: 10.3390/ijms24021785.

Lithium chloride sensitivity connects the activity of PEX11 and RIM20 to the translation of PGM2 and other mRNAs with structured 5'-UTRs.

Jagadeesan S, Al-Gafari M, Hajikarimlou M, Takallou S, Moteshareie H, Tayabali A Mol Cell Biochem. 2022; 477(11):2643-2656.

PMID: 35598219 DOI: 10.1007/s11010-022-04466-5.

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