A Mutation That Increases the Activity of Nonsense Suppressors in Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Feb 16

PMID 345090

Citations 11

Authors

L Mindich

Affiliations

Soon will be listed here.

Abstract

We have isolated a new mutation, ups, that amplifies the suppressor activity of all the nonsense suppressors we have tested so far at low but not at high temperature. The properties of ups make it a very useful tool to improve the systems of temperature sensitive suppressors thus far described. ups maps between 25 to 27 min on the E. coli genetic map (Bachmann et al., 1976) and has no suppressor activity of its own. Its effects on translational fidelity are not influenced by mutations for ribosomal drug resistance. Thus, ups is different from ram which exhibits cooperative control of translation with other ribosomal proteins. The possible functions of ups in the cell are discussed.

Citing Articles

A mutant allele of the SUP45 (SAL4) gene of Saccharomyces cerevisiae shows temperature-dependent allosuppressor and omnipotent suppressor phenotypes.

Stansfield I, Akhmaloka , Tuite M Curr Genet. 1995; 27(5):417-26.

PMID: 7586027 DOI: 10.1007/BF00311210.

Functions of the gene products of Escherichia coli.

Riley M Microbiol Rev. 1993; 57(4):862-952.

PMID: 7508076 PMC: 372942. DOI: 10.1128/mr.57.4.862-952.1993.

High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli.

Zengel J, Lindahl L J Bacteriol. 1981; 145(1):459-65.

PMID: 7007324 PMC: 217294. DOI: 10.1128/jb.145.1.459-465.1981.

Linkage map of Escherichia coli K-12, edition 6.

Bachmann B, Low K Microbiol Rev. 1980; 44(1):1-56.

PMID: 6997720 PMC: 373233. DOI: 10.1128/mr.44.1.1-56.1980.

High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: isolation of strains with suppressor-enhancing mutations.

Oeschger M, Oeschger N, Wiprud G, Woods S Mol Gen Genet. 1980; 177(4):545-52.

PMID: 6991863 DOI: 10.1007/BF00272662.

References

Rosset R, GORINI L . A ribosomal ambiguity mutation. J Mol Biol. 1969; 39(1):95-112. DOI: 10.1016/0022-2836(69)90336-2. View

Topisirovic L, Villarroel R, de Wilde M, Herzog A, Cabezon T, Bollen A . Translational fidelity in Escherichia coli: contrasting role of neaA and ramA gene products in the ribosome functioning. Mol Gen Genet. 1977; 151(1):89-94. DOI: 10.1007/BF00446917. View

Smith J, Barnett L, Brenner S, Russell R . More mutant tyrosine transfer ribonucleic acids. J Mol Biol. 1970; 54(1):1-14. DOI: 10.1016/0022-2836(70)90442-0. View

Gallucci E, Pacchetti G, Zangrossi S . Genetic studies on temperature sensitive nonsense suppression. Mol Gen Genet. 1970; 106(4):362-70. DOI: 10.1007/BF00324053. View

Menninger J, Walker C, Tan P . Studies on the metabolic role of peptidyl-tRNA hydrolase. I. Properties of a mutant E. coli with temperature-sensitive peptidyl-tRNA hydrolase. Mol Gen Genet. 1973; 121(4):307-24. DOI: 10.1007/BF00433230. View

Mindich L, Cohen J, Weisburd M . Isolation of nonsense suppressor mutants in Pseudomonas. J Bacteriol. 1976; 126(1):177-82. PMC: 233272. DOI: 10.1128/jb.126.1.177-182.1976. View

Sparling P, Blackman E . Mutation to erythromycin dependence in Escherichia coli K-12. J Bacteriol. 1973; 116(1):74-83. PMC: 246393. DOI: 10.1128/jb.116.1.74-83.1973. View

Spudich J, Horn V, Yanofsky C . On the production of deletions in the chromosome of Escherichia coli. J Mol Biol. 1970; 53(1):49-67. DOI: 10.1016/0022-2836(70)90045-8. View

Chelala C, Margolin P . Effects of deletions on cotransduction linkage in Salmonella typhimurium: evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Mol Gen Genet. 1974; 131(2):97-112. DOI: 10.1007/BF00266146. View

10.

de Wilde M, Cabezon T, Villarroel R, Herzog A, Bollen A . Cooperative control of translation fidelity by ribosomal proteins in Escherichia coli. I. Properties of ribosomal mutants whose resistance to neamine is the cumulative effect of two distinct mutations. Mol Gen Genet. 1975; 142(1):19-33. DOI: 10.1007/BF00268752. View

11.

Russell R, Abelson J, Landy A, Gefter M, Brenner S, Smith J . Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970; 47(1):1-13. DOI: 10.1016/0022-2836(70)90397-9. View

12.

Kuhn J, SOMERVILLE R . Mutant strains of Escherichia coli K12 that use D-amino acids. Proc Natl Acad Sci U S A. 1971; 68(10):2484-7. PMC: 389449. DOI: 10.1073/pnas.68.10.2484. View

13.

GORINI L . Ribosomal discrimination of tRNAs. Nat New Biol. 1971; 234(52):261-4. DOI: 10.1038/newbio234261a0. View

14.

Cabezon T, Herzog A, de Wilde M, Villarroel R, Bollen A . Cooperative control of translational fidelity by ribosomal proteins in Escherichia coli. III. A ram mutation in the structural gene for protein S5 (rpx E). Mol Gen Genet. 1976; 144(1):59-62. DOI: 10.1007/BF00277305. View

15.

Biswas D, GORINI L . Restriction, de-restriction and mistranslation in missense suppression. Ribosomal discrimination of transfer RNA's. J Mol Biol. 1972; 64(1):119-34. DOI: 10.1016/0022-2836(72)90324-5. View

16.

Nagata T, Horiuchi T . An amber dna mutant of Escherichia coli K12 affecting DNA ligase. J Mol Biol. 1974; 87(2):369-73. DOI: 10.1016/0022-2836(74)90156-9. View

17.

Nagata T, Horiuchi T . Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12. Mol Gen Genet. 1973; 123(1):77-88. DOI: 10.1007/BF00282991. View

18.

Garen A, GAREN S, WILHELM R . Suppressor genes for nonsense mutations. I. The Su-1, Su-2 and Su-3 genes of Escherichia coli. J Mol Biol. 1965; 14(1):167-78. DOI: 10.1016/s0022-2836(65)80238-8. View

19.

Strigini P, Brickman E . Analysis of specific misreading in Escherichia coli. J Mol Biol. 1973; 75(4):659-72. DOI: 10.1016/0022-2836(73)90299-4. View

20.

Studier F . Bacteriophage T7. Science. 1972; 176(4033):367-76. DOI: 10.1126/science.176.4033.367. View