Position Effect on Expression of Dsd Genes Cloned Onto Multicopy Plasmids

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Apr 1

PMID 6989803

Citations 5

Authors

A M Carothers

M C Heincz

E MCFALL

Affiliations

Soon will be listed here.

Abstract

In the D-serine deaminase system of Escherichia coli, which is regulated by positive control, we have fouand a complete lack of trans activation in vivo with multicopy dsd hybrid plasmids. A PLASmid carrying the regulatory gene, dsdC+, did not promote expression of chromosomal dsdCO+A+ loci, nor did a chromosomal dsdC+ gene promote expression of plasmid-borne dsdC delta O+A+ (dsd regulatory gene negative) restriction fragments. However, hybrid plasmids that comprise the entire dsd system (dsdC+O+A+) are highly inducible for the enzyme. These dsd hybrid plasmid deoxyribonucleic acids functioned well as templates in the in vitro coupled transcription-translation system. In vitro-synthesized dsdC+ protein promoted expression of the dsdA+ operation efficiently. Exogenously purified dsdC+ protein also activated expression of several dsdC delta O+A+ plasmid deoxyribonucleic acid templates in vitro. An explanation that reconciles these results with previous dominance studies is presented.

Citing Articles

DNA sequences of the D-serine deaminase control region and N-terminal portion of the structural gene.

MCFALL E, Runkel L J Bacteriol. 1983; 154(3):1508-12.

PMID: 6343359 PMC: 217637. DOI: 10.1128/jb.154.3.1508-1512.1983.

Physical mapping of the Escherichia coli D-serine deaminase region: contiguity of the dsd structural and regulatory genes.

Carothers A, MCFALL E, Palchaudhuri S J Bacteriol. 1980; 142(1):174-84.

PMID: 6246062 PMC: 293924. DOI: 10.1128/jb.142.1.174-184.1980.

Purification and characterization of D-serine deaminase activator protein.

Heincz M, Bornstein S, MCFALL E J Bacteriol. 1984; 160(1):42-9.

PMID: 6090429 PMC: 214678. DOI: 10.1128/jb.160.1.42-49.1984.

cis-acting proteins.

MCFALL E J Bacteriol. 1986; 167(2):429-32.

PMID: 3015868 PMC: 212905. DOI: 10.1128/jb.167.2.429-432.1986.

Effects of structural changes in the dsdA-dsdC intergenic region on D-serine deaminase synthesis.

MCFALL E, Nikam S, Palchaudhuri S J Bacteriol. 1991; 173(3):1161-7.

PMID: 1899415 PMC: 207237. DOI: 10.1128/jb.173.3.1161-1167.1991.

References

MCFALL E . Mapping of the d-serine deaminase region in Escherichia coli K-12. Genetics. 1967; 55(1):91-9. PMC: 1211375. DOI: 10.1093/genetics/55.1.91. View

MCFALL E . Dominance studies with stable merodiploids in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1967; 94(6):1982-8. PMC: 276930. DOI: 10.1128/jb.94.6.1982-1988.1967. View

MCFALL E . "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1967; 94(6):1989-93. PMC: 276931. DOI: 10.1128/jb.94.6.1989-1993.1967. View

Clewell D, Helinski D . Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol. 1972; 110(3):1135-46. PMC: 247537. DOI: 10.1128/jb.110.3.1135-1146.1972. View

Zubay G . In vitro synthesis of protein in microbial systems. Annu Rev Genet. 1973; 7:267-87. DOI: 10.1146/annurev.ge.07.120173.001411. View

Hershfield V, Boyer H, Yanofsky C, Lovett M, Helinski D . Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974; 71(9):3455-9. PMC: 433792. DOI: 10.1073/pnas.71.9.3455. View

Bloom F, MCFALL E, Young M, Carothers A . Positive control in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1975; 121(3):1092-101. PMC: 246040. DOI: 10.1128/jb.121.3.1092-1101.1975. View

Echols H, Court D, Green L . On the nature of cis-acting regulatory proteins and genetic organization in bacteriophage: the example of gene Q of bacteriophage lambda. Genetics. 1976; 83(1):5-10. PMC: 1213504. DOI: 10.1093/genetics/83.1.5. View

Clarke L, Carbon J . A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell. 1976; 9(1):91-9. DOI: 10.1016/0092-8674(76)90055-6. View

10.

Backman K, Ptashne M, Gilbert W . Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A. 1976; 73(11):4174-8. PMC: 431373. DOI: 10.1073/pnas.73.11.4174. View

11.

McCorkle G, Leathers T, Umbarger H . Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. Proc Natl Acad Sci U S A. 1978; 75(1):89-93. PMC: 411189. DOI: 10.1073/pnas.75.1.89. View

12.

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

13.

Heincz M, KELKER N, MCFALL E . Positive control of D-serine deaminase synthesis in vitro. Proc Natl Acad Sci U S A. 1978; 75(4):1695-9. PMC: 392405. DOI: 10.1073/pnas.75.4.1695. View

14.

Widera G, Gautier F, Lindenmaier W, Collins J . The expression of tetracycline resistance after insertion of foreign DNA fragments between the EcoRI and HindIII sites of the plasmid cloning vector pBR 322. Mol Gen Genet. 1978; 163(3):301-5. DOI: 10.1007/BF00271959. View

15.

Heincz M, MCFALL E . Role of the dsdC activator in regulation of D-serine deaminase synthesis. J Bacteriol. 1978; 136(1):96-103. PMC: 218636. DOI: 10.1128/jb.136.1.96-103.1978. View

16.

Eckhardt T . A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978; 1(4):584-8. DOI: 10.1016/0147-619x(78)90016-1. View

17.

Wallace L, Wilcox G . Regulation of the L-arabinose operon in strains of Escherichia coli containing ColE1-ara hybrid plasmids. Mol Gen Genet. 1979; 173(3):323-31. DOI: 10.1007/BF00268643. View

18.

Carothers A, MCFALL E, Palchaudhuri S . Physical mapping of the Escherichia coli D-serine deaminase region: contiguity of the dsd structural and regulatory genes. J Bacteriol. 1980; 142(1):174-84. PMC: 293924. DOI: 10.1128/jb.142.1.174-184.1980. View