Effects of Structural Changes in the DsdA-dsdC Intergenic Region on D-serine Deaminase Synthesis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Feb 1

PMID 1899415

Citations 2

Authors

E MCFALL

S S Nikam

S Palchaudhuri

Affiliations

Soon will be listed here.

Abstract

Single-base-pair changes well upstream of its transcription initiation site resulted in partially to fully constitutive expression of the D-serine deaminase structural gene, dsdA, independently of the cyclic AMP-cyclic AMP-binding protein complex and of the specific D-serine deaminase activator protein. These promoter mutations appear to define a consensus sequence that is repeated several times. Basal expression of dsdA+ was also strongly enhanced by subcloning on multicopy plasmids, by the DNA gyrase inhibitor novobiocin, and in dsdC(Con) mutants by increasing growth temperature. These results suggest that activation of dsdA+ expression by the dsdC-encoded protein involves distortion of promoter DNA. A dsdA translation start at bp -731 was verified by subcloning of dsdC+. Plasmid-specified activator at a high concentration interfered with chromosomal dsdC(Con) expression, and the interference was enhanced by deletion of most of the intergenic region from the plasmid. Even at a high concentration, however, plasmid-specified activator did not activate expression of chromosomal dsdA+, and in one case it was actually repressive. These results confirm the strong cis tropism of plasmid-specified dsdC-encoded protein and suggest that it is mediated by multiple sites in the dsdA-dsdC intergenic region.

Citing Articles

D-serine deaminase is a stringent selective marker in genetic crosses.

Maas W, Maas R, MCFALL E J Bacteriol. 1995; 177(2):459-61.

PMID: 7814336 PMC: 176610. DOI: 10.1128/jb.177.2.459-461.1995.

Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.

MCFALL E, Valentin-Hansen P J Bacteriol. 1995; 177(22):6456-61.

PMID: 7592420 PMC: 177495. DOI: 10.1128/jb.177.22.6456-6461.1995.

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