Positive Control in the D-serine Deaminase System of Escherichia Coli K-12

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 Mar 1

PMID 1090589

Citations 24

Authors

F R Bloom

E MCFALL

M C Young

A M Carothers

Affiliations

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Abstract

Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator negative) mutations. The mutations lie in the dsdC region, are cis and trans recessive to dsdC+, and give rise to revertants of novel regulatory phenotype. The second class consists of Dsdase-negative lysogens in which the phenotype is the result of the integration of lambdac1857 Sam7 into the dsdC region. Lambda lysates derived from two of the Dsdase-negative lysogens can transduce the structural gene for Dsdase (dsdA) but not the dsdC region. The dsdC+ gene product had no repressor effect on constitutive synthesis in a strain containing a dsdO (initiator constitutive) and a dsdC- mutation. These and other findings indicate that control of Dsdase synthesis is strictly positive. The partial trans effect of the dsdC+ gene product on constitutive synthesis in dsdCc (regulator constitutive) strains can thus be explained by "subunit mixing" between active dsdCc subunits and dsdC+ subunits which are inactive in the absence of the inducer, D-serine. The order of genes in the dsd region is supN-dsdC-dsdP-dsdA-aroC.

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Position effect on expression of dsd genes cloned onto multicopy plasmids.

Carothers A, Heincz M, MCFALL E J Bacteriol. 1980; 142(1):185-90.

PMID: 6989803 PMC: 293926. DOI: 10.1128/jb.142.1.185-190.1980.

Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene.

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Identification and control of synthesis of the dsdC activator protein.

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PMID: 6296057 PMC: 221708. DOI: 10.1128/jb.153.2.872-877.1983.

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