Construction of Plasmids Carrying the CI Gene of Bacteriophage Lambda

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Nov 1

PMID 1069307

Citations 206

Authors

K Backman

M Ptashne

W Gilbert

Affiliations

Soon will be listed here.

Abstract

By techniques of recombination in vitro, we have constructed a plasmid bearing the repressor gene (cI) of bacteriophage lambda fused to the promoter of the lac operon. Strains carrying this plasmid overproduce lambda repressor. This functional cI gene was reconstituted by joining DNA fragments bearing different parts of that gene. Flush end fusion techniques, involving no sequence overlap, were necessary for the construction; in certain cases, the abutting of the DNA molecules bearing ends generated by different restriction endonucleases creates a sequence at the junction which is recognized by one of the restriction endonucleases.

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