Nova-ST: Nano-patterned Ultra-dense Platform for Spatial Transcriptomics

Overview

Journal Cell Rep Methods

Specialty Cell Biology

Date 2024 Aug 7

PMID 39111312

Authors

Suresh Poovathingal

Kristofer Davie

Lars E Borm

Roel Vandepoel

Nicolas Poulvellarie

Annelien Verfaillie

Nikky Corthout

Stein Aerts

Affiliations

Soon will be listed here.

Abstract

Spatial transcriptomics workflows using barcoded capture arrays are commonly used for resolving gene expression in tissues. However, existing techniques are either limited by capture array density or are cost prohibitive for large-scale atlasing. We present Nova-ST, a dense nano-patterned spatial transcriptomics technique derived from randomly barcoded Illumina sequencing flow cells. Nova-ST enables customized, low-cost, flexible, and high-resolution spatial profiling of large tissue sections. Benchmarking on mouse brain sections demonstrates significantly higher sensitivity compared to existing methods at a reduced cost.

Citing Articles

Seq-Scope-eXpanded: Spatial Omics Beyond Optical Resolution.

Anacleto A, Cheng W, Feng Q, Cho C, Hwang Y, Kim Y bioRxiv. 2025; .

PMID: 39975076 PMC: 11838548. DOI: 10.1101/2025.02.04.636355.

Protocol for high-resolution 3D spatial transcriptomics using Open-ST.

Schott M, Leon-Perinan D, Splendiani E, Ferretti E, Macino G, Karaiskos N STAR Protoc. 2024; 6(1):103521.

PMID: 39708325 PMC: 11731217. DOI: 10.1016/j.xpro.2024.103521.

Seq-Scope: repurposing Illumina sequencing flow cells for high-resolution spatial transcriptomics.

Kim Y, Cheng W, Cho C, Hwang Y, Si Y, Park A Nat Protoc. 2024; 20(3):643-689.

PMID: 39482362 PMC: 11896753. DOI: 10.1038/s41596-024-01065-0.

Nova-ST: Nano-patterned ultra-dense platform for spatial transcriptomics.

Poovathingal S, Davie K, Borm L, Vandepoel R, Poulvellarie N, Verfaillie A Cell Rep Methods. 2024; 4(8):100831.

PMID: 39111312 PMC: 11384075. DOI: 10.1016/j.crmeth.2024.100831.

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