Demuxafy: Improvement in Droplet Assignment by Integrating Multiple Single-cell Demultiplexing and Doublet Detection Methods

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2024 Apr 15

PMID 38622708

Authors

Drew Neavin

Anne Senabouth

Himanshi Arora

Jimmy Tsz Hang Lee

Aida Ripoll-Cladellas

Lude Franke

Shyam Prabhakar

Chun Jimmie Ye

Davis J McCarthy

Marta Mele

Martin Hemberg

Joseph E Powell

Affiliations

Soon will be listed here.

Abstract

Recent innovations in single-cell RNA-sequencing (scRNA-seq) provide the technology to investigate biological questions at cellular resolution. Pooling cells from multiple individuals has become a common strategy, and droplets can subsequently be assigned to a specific individual by leveraging their inherent genetic differences. An implicit challenge with scRNA-seq is the occurrence of doublets-droplets containing two or more cells. We develop Demuxafy, a framework to enhance donor assignment and doublet removal through the consensus intersection of multiple demultiplexing and doublet detecting methods. Demuxafy significantly improves droplet assignment by separating singlets from doublets and classifying the correct individual.

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