MicroRNA-200c Attenuates the Tumor-Infiltrating Capacity of Macrophages

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2022 Mar 26

PMID 35336722

Authors

Rebecca Raue

Ann-Christin Frank

Dominik C Fuhrmann

Patricia de la Cruz-Ojeda

Silvia Rosser

Rebekka Bauer

Giulia Cardamone

Andreas Weigert

Shahzad Nawaz Syed

Tobias Schmid

Bernhard Brune

Affiliations

Soon will be listed here.

Abstract

Macrophages constitute a major part of the tumor-infiltrating immune cells. Within the tumor microenvironment, they acquire an alternatively activated, tumor-supporting phenotype. Factors released by tumor cells are crucial for the recruitment of tumor-associated macrophages. In the present project, we aimed to understand the role of hsa-miR-200c-3p (miR-200c) in the interplay between tumor cells and macrophages. To this end, we employed a coculture system of MCF7 breast tumor cells and primary human macrophages and observed the transfer of miR-200c from apoptotic tumor cells to macrophages, which required intact CD36 receptor in macrophages. We further comprehensively determined miR-200c targets in macrophages by mRNA-sequencing and identified numerous migration-associated mRNAs to be downregulated by miR-200c. Consequently, miR-200c attenuated macrophage infiltration into 3-dimensional tumor spheroids. miR-200c-mediated reduction in infiltration further correlated with a miR-200c migration signature comprised of the four miR-200c-repressed, predicted targets PPM1F, RAB11FIB2, RDX, and MSN.

Citing Articles

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