Bifunctional Peptide That Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing

Overview

Journal ACS Chem Biol

Publisher American Chemical Society

Specialties Biochemistry
Biology

Date 2022 Jan 27

PMID 35084170

Authors

Sayani Chattopadhyay

Leandro B C Teixeira

Laura L Kiessling

Jonathan F McAnulty

Ronald T Raines

Affiliations

Soon will be listed here.

Abstract

Transforming growth factor-β (TGF-β) plays important roles in wound healing. The activity of TGF-β is initiated upon the binding of the growth factor to the extracellular domains of its receptors. We sought to facilitate the activation by clustering these extracellular domains. To do so, we used a known peptide that binds to TGF-β receptors without diminishing their affinity for TGF-β. We conjugated this peptide to a collagen-mimetic peptide that can anneal to the damaged collagen in a wound bed. We find that the conjugate enhances collagen deposition and wound closure in mice in a manner consistent with the clustering of TGF-β receptors. This strategy provides a means to upregulate the TGF-β signaling pathway without adding exogenous TGF-β and could inspire means to treat severe wounds.

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