Cytogenetic Studies on Cervus Elaphus. II. Synaptonemal Complexes and NOR Activity During Spermatogenesis

Overview

Journal Genetica

Specialties Cell Biology
Genetics

Date 1987 Oct 15

PMID 3506531

Authors

R Lunghi

M Rubini

D Goldoni

F Fontana

Affiliations

Soon will be listed here.

Abstract

The activity of the nucleolus organizer regions (NORs) in various stages of spermatogenesis of the red deer was studied with the silver staining technique. The Ag-stainability of the NORs, an indicator of the transcriptional activity of the r-RNA genes, is present during the meiotic prophase until pachytene and is absent during the remainder of the meiotic prophase, to reappear during the first stages of spermiogenesis and then disappear again during the elongation phase of the spermatids. The development and behaviour of synaptonemal complexes (SCs) of primary spermatocytes were also studied with the silver staining technique under the light microscope. The organization of SC is similar to that of other mammalian species. The pairing behaviour of the axes of the sex chromosomes is described.

References

Rouyer F, Simmler M, Johnsson C, Vergnaud G, Cooke H, Weissenbach J . A gradient of sex linkage in the pseudoautosomal region of the human sex chromosomes. Nature. 1986; 319(6051):291-5. DOI: 10.1038/319291a0. View

Howell W, Black D . Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980; 36(8):1014-5. DOI: 10.1007/BF01953855. View

Geremia R, DAgostino A, MONESI V . Biochemical evidence of haploid gene activity in spermatogenesis of the mouse. Exp Cell Res. 1978; 111(1):23-30. DOI: 10.1016/0014-4827(78)90232-x. View

Tres L . Extensive pairing of the XY bivalent in mouse spermatocytes as visualized by whole-mount electron microscopy. J Cell Sci. 1977; 25:1-15. DOI: 10.1242/jcs.25.1.1. View

MOSES M . Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). II. Morphology of the XY pair in spread preparations. Chromosoma. 1977; 60(2):127-37. DOI: 10.1007/BF00288460. View

Evans E, BRECKON G, Ford C . AN AIR-DRYING METHOD FOR MEIOTIC PREPARATIONS FROM MAMMALIAN TESTES. Cytogenetics. 1964; 3:289-94. DOI: 10.1159/000129818. View

Pathak S, Hsu T . Silver-stained structures in mammalian meiotic prophase. Chromosoma. 1979; 70(2):195-203. DOI: 10.1007/BF00288406. View

Pathak S, Elder F . Silver-stained accessory structures on human sex chromosomes. Hum Genet. 1980; 54(2):171-5. DOI: 10.1007/BF00278967. View

Pathak S, Lau Y, Drwinga H . Observations on the synaptonemal complex in Armenian hamster spermatocytes by light microscopy. Chromosoma. 1979; 73(1):53-60. DOI: 10.1007/BF00294844. View

10.

Dresser M, MOSES M . Silver staining of synaptonemal complexes in surface spreads for light and electron microscopy. Exp Cell Res. 1979; 121(2):416-9. DOI: 10.1016/0014-4827(79)90023-5. View

11.

Schmid M, Loser C, Schmidtke J, Engel W . Evolutionary conservation of a common pattern of activity of nucleolus organizers during spermatogenesis in vertebrates. Chromosoma. 1982; 86(2):149-79. DOI: 10.1007/BF00288674. View

12.

Fletcher J . Light microscope analysis of meiotic prophase chromosomes by silver staining. Chromosoma. 1979; 72(2):241-8. DOI: 10.1007/BF00293237. View

13.

Schmid M, Muller H, Stasch S, Engel W . Silver staining of nucleolus organizer regions during human spermatogenesis. Hum Genet. 1983; 64(4):363-70. DOI: 10.1007/BF00292368. View

14.

Forejt J, Goetz P . Synaptonemal complexes of mouse and human pachytene chromosomes visualized by silver staining in air-dried preparations. Chromosoma. 1979; 73(2):255-61. DOI: 10.1007/BF00331576. View

15.

Stahl A, Luciani J, Devictor M, Capodano A, Gagne R . Constitutive heterochromatin and micronucleoli in the human oocyte at the diplotene stage. Humangenetik. 1975; 26(4):315-27. DOI: 10.1007/BF00285383. View

16.

Hofgartner F, Schmid M, Krone W, Zenzes M, Engel W . Pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining. Chromosoma. 1979; 71(2):197-216. DOI: 10.1007/BF00292823. View

17.

Buys C, Osinga J . Abundance of protein-bound sulfhydryl and disulfide groups at chromosomal nucleolus organizing regions: a cytochemical study on the selective silver staining of NORs. Chromosoma. 1980; 77(1):1-11. DOI: 10.1007/BF00292037. View

18.

MILLER O, Miller D, Dev V, Tantravahi R, Croce C . Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. Proc Natl Acad Sci U S A. 1976; 73(12):4531-5. PMC: 431529. DOI: 10.1073/pnas.73.12.4531. View

19.

Meredith R . A simple method for preparing meiotic chromosomes from mammalian testis. Chromosoma. 1969; 26(3):254-8. DOI: 10.1007/BF00326520. View

20.

Miller D, Dev V, Tantravahi R, MILLER O . Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp Cell Res. 1976; 101(2):235-43. DOI: 10.1016/0014-4827(76)90373-6. View