Light Microscope Analysis of Meiotic Prophase Chromosomes by Silver Staining

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1979 Apr 30

PMID 88315

Citations 50

Authors

J M Fletcher

Affiliations

Soon will be listed here.

Abstract

A method is described for the silver staining of the synaptonemal complex in surface-spread mammalian spermatocytes for light microscope examination. The method is quick, reliable, of broad applicability, and provides a means of making karyotype analysis at meiotic prophase. Many hundreds of suitable cells can be examined in an average preparation in a relatively short space of time. It has so far been applied only to mammalian spermatocytes, but could be used for karyotype analysis in oocytes of mammals and also applied to gonocytes of non-mammalian species.

Citing Articles

Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse.

Beyret E, Lin H Dev Biol. 2011; 355(2):215-26.

PMID: 21539824 PMC: 3443393. DOI: 10.1016/j.ydbio.2011.04.021.

Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads.

Belonogova N, Karamysheva T, Biltueva L, Perepelov E, Minina J, Polyakov A Chromosome Res. 2006; 14(6):673-9.

PMID: 16964574 DOI: 10.1007/s10577-006-1079-9.

Variation in meiotic recombination frequencies among human males.

Sun F, Trpkov K, Rademaker A, Ko E, Martin R Hum Genet. 2004; 116(3):172-8.

PMID: 15578224 DOI: 10.1007/s00439-004-1215-6.

Reduced meiotic fitness in hybrids with heterozygosity for heterochromatin in the speciating Mus terricolor complex.

Sharma T, Bardhan A, Bahadur M J Biosci. 2003; 28(2):189-98.

PMID: 12711811 DOI: 10.1007/BF02706218.

Male-biased distribution of the human Y chromosomal genes SRY and ZFY in the lizard Calotes versicolor, which lacks sex chromosomes and temperature-dependent sex determination.

Ganesh S, Mohanty J, Raman R Chromosome Res. 1997; 5(6):413-9.

PMID: 9364943 DOI: 10.1023/a:1018452526903.

References

Counce S, Meyer G . Differentiation of the synaptonemal complex and the kinetochore in Locusta spermatocytes studied by whole mount electron microscopy. Chromosoma. 1973; 44(2):231-53. DOI: 10.1007/BF00329119. View

Tres L . Extensive pairing of the XY bivalent in mouse spermatocytes as visualized by whole-mount electron microscopy. J Cell Sci. 1977; 25:1-15. DOI: 10.1242/jcs.25.1.1. View

Ferguson-Smith M, Page B . Pachytene analysis in a human reciprocal (10;11) translocation. J Med Genet. 1973; 10(3):282-7. PMC: 1013034. DOI: 10.1136/jmg.10.3.282. View

MOSES M . Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). II. Morphology of the XY pair in spread preparations. Chromosoma. 1977; 60(2):127-37. DOI: 10.1007/BF00288460. View

Quack B, Noel B . The XY chromosome pair in mouse and human spermatocytes, visualised by silver staining. Nature. 1977; 267(5610):431-3. DOI: 10.1038/267431a0. View

CHANDLEY A, Jones R, Dott H, Allen W, Short R . Meiosis in interspecific equine hybrids. I. The male mule (Equus asinus X E. caballus) and hinny (E. caballus X E. asinus). Cytogenet Cell Genet. 1974; 13(4):330-41. DOI: 10.1159/000130284. View

Evans E, BRECKON G, Ford C . AN AIR-DRYING METHOD FOR MEIOTIC PREPARATIONS FROM MAMMALIAN TESTES. Cytogenetics. 1964; 3:289-94. DOI: 10.1159/000129818. View

Miller D, MILLER O . Chromosome mapping in the mouse, fluorescence banding techniques permit assignment of most genetic linkage groups. Science. 1972; 178(4064):949-55. DOI: 10.1126/science.178.4064.949. View

MOSES M, Russel L, Cacheiro N . Mouse chromosome translocations: visualization and analysis by electron microscopy of the synaptonemal complex. Science. 1977; 196(4292):892-4. DOI: 10.1126/science.870966. View

10.

MOSES M . Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). I. Morphology of the autosomal complement in spread preparations. Chromosoma. 1977; 60(2):99-125. DOI: 10.1007/BF00288459. View

11.

Esponda P, Stockert J . Localization of the synaptonemal complex under the light microscope. Chromosoma. 1978; 68(1):83-90. DOI: 10.1007/BF00330374. View

12.

Ohno S, Kaplan W, Kinosita R . Heterochromatic regions and nucleolus organizers in chromosomes of the mouse, Mus musculus. Exp Cell Res. 1957; 13(2):358-64. DOI: 10.1016/0014-4827(57)90015-0. View

13.

Elsevier S, Ruddle F . Location of genes coding for 18S and 28S ribosomal RNA within the genome of Mus musculus. Chromosoma. 1975; 52(3):219-28. DOI: 10.1007/BF00332112. View

14.

MOSES M, Counce S, Paulson D . Synaptonemal complex complement of man in spreads of spermatocytes, with details of the sex chromosome pair. Science. 1975; 187(4174):363-5. View

15.

HUNGERFORD D, Mellman W, Balaban G, LaBadie G, Messatzzia L, Haller G . Chromosome structure and function in man. 3. Pachytene analysis and indentification of the supernumerary chromosome in a case of Down's syndrome (mongolism). Proc Natl Acad Sci U S A. 1970; 67(1):221-4. PMC: 283191. DOI: 10.1073/pnas.67.1.221. View

16.

HUNGERFORD D, Hungerford A . Chromosome structure and function in man. VI. Pachytene chromomere maps of 16, 17 and 18; pachytene as a reference standard for metaphase banding. Cytogenet Cell Genet. 1978; 21(4):212-30. DOI: 10.1159/000130898. View