Expression of Human and Suppression of Mouse Nucleolus Organizer Activity in Mouse-human Somatic Cell Hybrids

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Dec 1

PMID 1070003

Citations 105

Authors

O J MILLER

D A Miller

V G Dev

R Tantravahi

C M Croce

Affiliations

Soon will be listed here.

Abstract

Most mouse-human somatic cell hybrids show preferential loss of human chromosomes, absence of human 28S ribosomal RNA, and suppression of human nucleolus organizer activity, as visualized by the Ag-AS silver histochemical stain. In contrast, the mouse-human hybrids studied here show preferential loss of mouse chromosomes. The hybrids were made by fusion of HT-1080-6TG human fibrosarcoma cells with BALB/c mouse peritoneal macrophages or strain 129 mouse teratocarcinoma cells. The Ag-AS staining method shows nucleolus organizer activity of chromosomes 13, 14, 15, 21 (rarely), and 22 in the human parent and chromosomes 12, 15, 16 (rarely), and 18 in the BALB/c mouse parent. In the hybrid cells the human nucleolus organizer regions are active, as shown by Ag-AS staining and involvement in "satellite association." The mouse nucleolus organizer regions are not stained by the Ag-AS method even though mouse chromosomes 12, 15, and 18 are present in the BALB/c hybrids and at least one copy of each mouse chromosome is present in the teratocarcinoma-derived hybrids. Thus, in these mouse-human hybrids, unlike those that lose human chromosomes, only human nucleolus organizer activity is expressed, and mouse nucleolus organizer activity is suppressed.

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References

Hsu T, Spirito S, Pardue M . Distribution of 18+28S ribosomal genes in mammalian genomes. Chromosoma. 1975; 53(1):25-36. DOI: 10.1007/BF00329388. View

Mintz B, Illmensee K . Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc Natl Acad Sci U S A. 1975; 72(9):3585-9. PMC: 433040. DOI: 10.1073/pnas.72.9.3585. View

Sayavedra M, Eliceiri G . Regulation of species-specific ribosomal RNA in a somatic hybrid cell. Biochim Biophys Acta. 1975; 378(2):216-20. DOI: 10.1016/0005-2787(75)90109-4. View

BRAMWELL M, HANDMAKER S . Ribosomal RNA synthesis in human-mouse hybrid cells. Biochim Biophys Acta. 1971; 232(3):580-3. DOI: 10.1016/0005-2787(71)90611-3. View

Eliceiri G, Green H . Ribosomal RNA synthesis in human-mouse hybrid cells. J Mol Biol. 1969; 41(2):253-60. DOI: 10.1016/0022-2836(69)90390-8. View

COHN Z, Benson B . THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965; 121:153-70. PMC: 2137972. DOI: 10.1084/jem.121.1.153. View

LITTLEFIELD J . SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964; 145(3633):709-10. DOI: 10.1126/science.145.3633.709. View

Ohno S, Trujillo J, Kaplan W, Kinosita R . Nucleolus-organisers in the causation of chromosomal anomalies in man. Lancet. 1961; 2(7194):123-6. DOI: 10.1016/s0140-6736(61)92647-2. View

Miller D, Dev V, Tantravahi R, MILLER O . Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp Cell Res. 1976; 101(2):235-43. DOI: 10.1016/0014-4827(76)90373-6. View

10.

Tantravahi R, Miller D, Dev V, MILLER O . Detection of nucleolus organizer regions in chromosomes of human, chimpanzee, gorilla, orangutan and gibbon. Chromosoma. 1976; 56(1):15-27. DOI: 10.1007/BF00293725. View

11.

Croce C . Loss of mouse chromosomes in somatic cell hybrids between HT-1080 human fibrosarcoma cells and mouse peritioneal macrophages. Proc Natl Acad Sci U S A. 1976; 73(9):3248-52. PMC: 430994. DOI: 10.1073/pnas.73.9.3248. View

12.

Croce C, KOPROWSKI H, Eagle H . Effect of environmental pH on the efficiency of cellular hybridization. Proc Natl Acad Sci U S A. 1972; 69(7):1953-6. PMC: 426839. DOI: 10.1073/pnas.69.7.1953. View

13.

Weiss M, Green H . Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc Natl Acad Sci U S A. 1967; 58(3):1104-11. PMC: 335754. DOI: 10.1073/pnas.58.3.1104. View

14.

Rasheed S, Nelson-Rees W, Toth E, ARNSTEIN P, Gardner M . Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer. 1974; 33(4):1027-33. DOI: 10.1002/1097-0142(197404)33:4<1027::aid-cncr2820330419>3.0.co;2-z. View

15.

Howell W, Denton T, Diamond J . Differential staining of the satellite regions of human acrocentric chromosomes. Experientia. 1975; 31(2):260-2. DOI: 10.1007/BF01990741. View

16.

Goodpasture C, Bloom S . Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining. Chromosoma. 1975; 53(1):37-50. DOI: 10.1007/BF00329389. View

17.

Scheer U, Trendelenburg M, Franke W . Regulation of transcription of genes of ribosomal rna during amphibian oogenesis. A biochemical and morphological study. J Cell Biol. 1976; 69(2):465-89. PMC: 2109682. DOI: 10.1083/jcb.69.2.465. View

18.

Marshall C . Synthesis of ribosomal RNA in synkaryons and heterokaryons formed between human and rodent cells. J Cell Sci. 1975; 17(3):307-25. DOI: 10.1242/jcs.17.3.307. View

19.

Henderson A, Warburton D, Atwood K . Location of ribosomal DNA in the human chromosome complement. Proc Natl Acad Sci U S A. 1972; 69(11):3394-8. PMC: 389778. DOI: 10.1073/pnas.69.11.3394. View

20.

Eliceiri G . The ribosomal RNA of hamster-mouse hybrid cells. J Cell Biol. 1972; 53(1):177-84. PMC: 2108706. DOI: 10.1083/jcb.53.1.177. View