Controlled Silver-staining of Nucleolus Organizer Regions with a Protective Colloidal Developer: a 1-step Method

Overview

Journal Experientia

Specialty Science

Date 1980 Aug 15

PMID 6160049

Citations 568

Authors

W M Howell

D A Black

Affiliations

Soon will be listed here.

Abstract

A 1-step silver-staining technique, requiring only 2 min to perform, is described for the differential staining of nucleolus organizer regions. A protective colloidal developer is used to control the reduction of the silver.

Citing Articles

Evolutionary dynamics of the B chromosomes in the fish species Valenciennes, 1837 of the Paraná River Basin.

Penitente M, Goes C, Dos Santos R, Utsunomia R, Foresti F, Porto-Foresti F Comp Cytogenet. 2025; 19:1-12.

PMID: 39882391 PMC: 11773350. DOI: 10.3897/compcytogen.19.135127.

A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism.

Haerter C, Viana P, Takagui F, Tonello S, Margarido V, Blanco D Genet Mol Biol. 2025; 48(1):e20240071.

PMID: 39873589 PMC: 11789463. DOI: 10.1590/1678-4685-GMB-2024-0071.

The Observation of Meiotic Union Behavior of Gametophytes Provides a New Basis for Ploidy of .

Ma K, Yang Y, Li Y, Li C, Li T, Ma H Animals (Basel). 2025; 15(2.

PMID: 39858139 PMC: 11758287. DOI: 10.3390/ani15020140.

A Detailed Karyological Investigation of three Endemic Cobitis Linnaeus, 1758 Species (Teleostei, Cobitidae) in Anatolia, Türkiye.

Unal Karakus S, Gaffaroglu M, Karasu Ayata M, Knytl M Cytogenet Genome Res. 2024; 164(5-6):243-256.

PMID: 39622218 PMC: 11825084. DOI: 10.1159/000542804.

Comparative karyotypic study of fifteen cyprinids (Cyprinidae, Cyprininae) species. An insight into the chromosomal evolution of the tribe Systomini.

Buasriyot P, Sassi F, Donbundit N, Supiwong W, Kaewsri S, Jumrusthanasan S PLoS One. 2024; 19(7):e0305828.

PMID: 39024404 PMC: 11257272. DOI: 10.1371/journal.pone.0305828.

References

Howell W, Denton T, Diamond J . Differential staining of the satellite regions of human acrocentric chromosomes. Experientia. 1975; 31(2):260-2. DOI: 10.1007/BF01990741. View

Howell W, Black D . A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes. Hum Genet. 1978; 43(1):53-6. DOI: 10.1007/BF00396478. View

Bloom S, Goodpasture C . An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. Hum Genet. 1976; 34(2):199-206. DOI: 10.1007/BF00278889. View

Denton T, Howell W, Barrett J . Human nucleolar organizer chromosomes: satellite associations. Chromosoma. 1976; 55(1):81-4. DOI: 10.1007/BF00288330. View

Goodpasture C, Bloom S . Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining. Chromosoma. 1975; 53(1):37-50. DOI: 10.1007/BF00329389. View