The Emerging Landscape of Single-molecule Protein Sequencing Technologies

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2021 Jun 8

PMID 34099939

Citations 126

Authors

Javier Antonio Alfaro

Peggy Bohlander

Mingjie Dai

Mike Filius

Cecil J Howard

Xander F van Kooten

Shilo Ohayon

Adam Pomorski

Sonja Schmid

Aleksei Aksimentiev

Eric V Anslyn

Georges Bedran

Chan Cao

Mauro Chinappi

Etienne Coyaud

Cees Dekker

Gunnar Dittmar

Nicholas Drachman

Rienk Eelkema

David Goodlett

Sebastien Hentz

Umesh Kalathiya

Neil L Kelleher

Ryan T Kelly

Zvi Kelman

Sung Hyun Kim

Bernhard Kuster

David Rodriguez-Larrea

Stuart Lindsay

Giovanni Maglia

Edward M Marcotte

John P Marino

Christophe Masselon

Michael Mayer

Patroklos Samaras

Kumar Sarthak

Lusia Sepiashvili

Derek Stein

Meni Wanunu

Mathias Wilhelm

Peng Yin

Amit Meller

Chirlmin Joo

Affiliations

Soon will be listed here.

Abstract

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

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