Naive Stem Cell Blastocyst Model Captures Human Embryo Lineage Segregation

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2021 May 6

PMID 33957081

Citations 101

Authors

Ayaka Yanagida

Daniel Spindlow

Jennifer Nichols

Anish Dattani

Austin Smith

Ge Guo

Affiliations

Soon will be listed here.

Abstract

Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.

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