Spatial Proteomic Approach to Characterize Skeletal Muscle Myofibers

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2020 Nov 30

PMID 33251806

Citations 7

Authors

Katherine M Fomchenko

Elise M Walsh

Xiaoping Yang

Rohan X Verma

Brian L Lin

Tim O Nieuwenhuis

Arun H Patil

Karen Fox-Talbot

Matthew N McCall

David A Kass

Avi Z Rosenberg

Marc K Halushka

Affiliations

Soon will be listed here.

Abstract

Skeletal muscle myofibers have differential protein expression resulting in functionally distinct slow- and fast-twitch types. While certain protein classes are well-characterized, the depth of all proteins involved in this process is unknown. We utilized the Human Protein Atlas (HPA) and the HPASubC tool to classify mosaic expression patterns of staining across 49,600 unique tissue microarray (TMA) images using a visual proteomic approach. We identified 2164 proteins with potential mosaic expression, of which 1605 were categorized as "likely" or "real." This list included both well-known fiber-type-specific and novel proteins. A comparison of the 1605 mosaic proteins with a mass spectrometry (MS)-derived proteomic dataset of single human muscle fibers led to the assignment of 111 proteins to fiber types. We additionally used a multiplexed immunohistochemistry approach, a multiplexed RNA-ISH approach, and STRING v11 to further assign or suggest fiber types of newly characterized mosaic proteins. This visual proteomic analysis of mature skeletal muscle myofibers greatly expands the known repertoire of twitch-type-specific proteins.

Citing Articles

Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix.

Dowling P, Gargan S, Zweyer M, Henry M, Meleady P, Swandulla D Eur J Transl Myol. 2024; 34(2).

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Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning.

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Simulated microgravity attenuates myogenesis and contractile function of 3D engineered skeletal muscle tissues.

Ren Z, Ahn E, Do M, Mair D, Monemianesfahani A, Lee P NPJ Microgravity. 2024; 10(1):18.

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Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology.

Dowling P, Swandulla D, Ohlendieck K Cells. 2023; 12(21).

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Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles.

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PMID: 36768735 PMC: 9916839. DOI: 10.3390/ijms24032415.

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