Single Muscle Fiber Proteomics Reveals Unexpected Mitochondrial Specialization

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2015 Feb 4

PMID 25643707

Citations 92

Authors

Marta Murgia

Nagarjuna Nagaraj

Atul S Deshmukh

Marlis Zeiler

Pasqua Cancellara

Irene Moretti

Carlo Reggiani

Stefano Schiaffino

Matthias Mann

Affiliations

Soon will be listed here.

Abstract

Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

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