Designed Heterochiral Blue Fluorescent Protein

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2020 Oct 28

PMID 33110966

Citations 1

Authors

Vivek Prakash

Ranjit Ranbhor

Vibin Ramakrishnan

Affiliations

Soon will be listed here.

Abstract

Diversification of chain stereochemistry offers a tremendous increase in protein design space. We have designed a minimal fluorescent protein, pregnant with β-(1-azulenyl)-l-alanine in the hydrophobic core of a heterotactic protein scaffold, employing automated design tools such as automated repetitive simulated annealing molecular dynamics and IDeAS. The designed heterochiral protein can be selectively excited at 342 nm, quite distant from the intrinsic fluorophore, and emits in the blue region. The structure and stability of the designed proteins were evaluated by established spectroscopic and calorimetric methods.

Citing Articles

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Ferrando J, Solomon L Life (Basel). 2021; 11(3).

PMID: 33802210 PMC: 7999464. DOI: 10.3390/life11030225.

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