Depletion of Microbiome-derived Molecules in the Host Using Genetics

Overview

Journal Science

Specialty Science

Date 2019 Dec 14

PMID 31831639

Citations 64

Authors

Chun-Jun Guo

Breanna M Allen

Kamir J Hiam

Dylan Dodd

Will Van Treuren

Steven Higginbottom

Kazuki Nagashima

Curt R Fischer

Justin L Sonnenburg

Matthew H Spitzer

Michael A Fischbach

Affiliations

Soon will be listed here.

Abstract

The gut microbiota produce hundreds of molecules that are present at high concentrations in the host circulation. Unraveling the contribution of each molecule to host biology remains difficult. We developed a system for constructing clean deletions in spp., the source of many molecules from the gut microbiome. By applying this method to the model commensal organism , we knocked out genes for 10 -derived molecules that accumulate in host tissues. In mice colonized by a for which the production of branched short-chain fatty acids was knocked out, we discovered that these microbial products have immunoglobulin A-modulatory activity.

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