Diversification of the Heat Shock Response by Helitron Transposable Elements

Overview

Journal Elife

Specialty Biology

Date 2019 Dec 12

PMID 31825311

Citations 17

Authors

Jacob M Garrigues

Brian V Tsu

Matthew D Daugherty

Amy E Pasquinelli

Affiliations

Soon will be listed here.

Abstract

Heat Shock Factor 1 (HSF-1) is a key regulator of the heat shock response (HSR). Upon heat shock, HSF-1 binds well-conserved motifs, called Heat Shock Elements (HSEs), and drives expression of genes important for cellular protection during this stress. Remarkably, we found that substantial numbers of HSEs in multiple species reside within Helitrons, a type of DNA transposon. Consistent with Helitron-embedded HSEs being functional, upon heat shock they display increased HSF-1 and RNA polymerase II occupancy and up-regulation of nearby genes in . Interestingly, we found that different genes appear to be incorporated into the HSR by species-specific Helitron insertions in and and by strain-specific insertions among different wild isolates of . Our studies uncover previously unidentified targets of HSF-1 and show that Helitron insertions are responsible for rewiring and diversifying the HSR.

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