In Vivo and Deletion Elucidates Roles for Autosomal Gene Regulation

Overview

Journal Elife

Specialty Biology

Date 2019 Nov 19

PMID 31738164

Citations 17

Authors

Daniel Andergassen

Zachary D Smith

Jordan P Lewandowski

Chiara Gerhardinger

Alexander Meissner

John L Rinn

Affiliations

Soon will be listed here.

Abstract

Recent evidence has determined that the conserved X chromosome mega-structures controlled by the and loci are not required for X chromosome inactivation (XCI) in cell lines. Here, we examined the in vivo contribution of these loci by generating mice carrying a single or double deletion of and . We found that these mutants are viable, fertile and show no defect in random or imprinted XCI. However, the lack of these elements results in many dysregulated genes on autosomes in an organ-specific manner. By comparing the dysregulated genes between the single and double deletion, we identified superloop, megadomain, and locus-dependent gene sets. The largest transcriptional effect was observed in all strains lacking the locus, indicating that this locus is the main driver for these autosomal expression signatures. Collectively, these findings suggest that these X-linked loci are involved in autosomal gene regulation rather than XCI biology.

Citing Articles

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