Clinical Outcomes of Transfer of Frozen and Thawed Single Blastocysts Derived from Nonpronuclear and Monopronuclear Zygotes

Overview

Journal Reprod Med Biol

Specialty Biology

Date 2019 Jul 18

PMID 31312107

Citations 11

Authors

Saki Hondo

Akane Arichi

Hirotaka Muramatsu

Naoki Omura

Kaori Ito

Hokuto Komine

Shiho Monzen

Naoko Mukai

Miyuki Endo

Serina Katase

Nobuyuki Kidera

Hiroshi Sasaki

Hideki Koi

Takeshi Yorimitsu

Motohiro Ohara

Toshihiro Kawamura

Yasufumi Shimizu

Affiliations

Soon will be listed here.

Abstract

Purpose: In assisted reproductive technology, normal zygotes are bipronuclear (2PN) during fertilization confirmation; however, sometimes, nonpronuclear zygotes (0PN) and monopronuclear zygotes (1PN) are found during routine observations.

Methods: To elucidate the clinical usefulness of -fertilized embryos, we investigated the rates of clinical pregnancy, live birth, miscarriage, and congenital abnormality after transfer of frozen-thawed 1PN- and 0PN-derived single blastocysts at Denentoshi Ladies Clinic, Kanagawa, Japan.

Results: The rates of pregnancy and live birth for 1PN-derived blastocysts obtained by conventional fertilization were 37.5% and 27.1%, respectively, which was not significantly different from those for 2PN-derived blastocysts; however, the rates for 0PN-derived blastocysts were significantly lower. The pregnancy and live birth rates for 0PN-derived embryos obtained by intracytoplasmic sperm injection (ICSI) were 45.7% and 34.8%, respectively, which was not significantly different from those for 2PN-derived blastocysts; however, the rates for 1PN-derived blastocysts were significantly lower (4.0% for both) than those for 2PN- and 0PN-derived blastocysts. No congenital abnormalities were found in infants resulting from transfer of 0PN- or 1PN-derived blastocysts.

Conclusions: Both 1PN- and 0PN-derived blastocysts can be used for embryo transfer; however, care should be taken in making decisions about 1PN-derived blastocysts, especially if they are obtained by ICSI.

Citing Articles

Developmental potential of non- and mono-pronuclear zygotes and associated clinical outcomes in IVF cycles.

Zhu M, Dong Q, Zhu Y, Le Y, Wang T, Zhou Y Front Endocrinol (Lausanne). 2024; 15:1361734.

PMID: 38532894 PMC: 10964251. DOI: 10.3389/fendo.2024.1361734.

Clinical outcomes of frozen-thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles.

Tong X, Jin J, Xue Y, Fang L, Zhu H, Jiang L Arch Gynecol Obstet. 2023; 308(3):1015-1022.

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A randomised control trial on oral dydrogesterone micronized vaginal progesterone pessary for luteal phase support in fertilization cycles.

Ikechebelu J, Dim C, Eleje G, Joe-Ikechebelu N, Okpala B, Okam P J Med Life. 2023; 16(1):62-69.

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Development of a Model Predicting the Outcome of Fertilization Cycles by a Robust Decision Tree Method.

Fu K, Li Y, Lv H, Wu W, Song J, Xu J Front Endocrinol (Lausanne). 2022; 13:877518.

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Does the cell number of 0PN embryos on day 3 affect pregnancy and neonatal outcomes following single blastocyst transfer?.

Chen C, Li W, Yin M, Li M, Wu L, Si J BMC Pregnancy Childbirth. 2022; 22(1):200.

PMID: 35279109 PMC: 8918324. DOI: 10.1186/s12884-022-04492-7.

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