Single Organelle Analysis to Characterize Mitochondrial Function and Crosstalk During Viral Infection

Overview

Journal Sci Rep

Specialty Science

Date 2019 Jun 13

PMID 31186476

Citations 11

Authors

Annika Schneider

Sandra Kurz

Katrin Manske

Marianne Janas

Mathias Heikenwalder

Thomas Misgeld

Michaela Aichler

Sebastian Felix Weissmann

Hans Zischka

Percy Knolle

Dirk Wohlleber

Affiliations

Soon will be listed here.

Abstract

Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.

Citing Articles

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