Electron Cryo-tomography Provides Insight into Procentriole Architecture and Assembly Mechanism

Overview

Journal Elife

Specialty Biology

Date 2019 Feb 12

PMID 30741631

Citations 22

Authors

Sam Li

Jose-Jesus Fernandez

Wallace F Marshall

David A Agard

Affiliations

Soon will be listed here.

Abstract

Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from . We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. The structure infers that POC1 is likely an integral component of A-C linker. Its conserved WD40 β-propeller domain provides attachment sites for other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly.

Citing Articles

The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography.

Li S, Fernandez J, Ruehle M, Howard-Till R, Fabritius A, Pearson C EMBO J. 2025; .

PMID: 39994484 DOI: 10.1038/s44318-025-00392-6.

An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity.

Sala C, Wurtz M, Atorino E, Neuner A, Partscht P, Hoffmann T Nat Commun. 2024; 15(1):9857.

PMID: 39543170 PMC: 11564547. DOI: 10.1038/s41467-024-54247-5.

The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.

Li S, Fernandez J, Ruehle M, Howard-Till R, Fabritius A, Pearson C bioRxiv. 2024; .

PMID: 39314311 PMC: 11419100. DOI: 10.1101/2024.09.09.612100.

Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.

Ruehle M, Li S, Agard D, Pearson C J Cell Biol. 2024; 223(8).

PMID: 38743010 PMC: 11094743. DOI: 10.1083/jcb.202311104.

Time-series reconstruction of the molecular architecture of human centriole assembly.

Laporte M, Gambarotto D, Bertiaux E, Bournonville L, Louvel V, Nunes J Cell. 2024; 187(9):2158-2174.e19.

PMID: 38604175 PMC: 11060037. DOI: 10.1016/j.cell.2024.03.025.

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