Structural Basis of the 9-fold Symmetry of Centrioles

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2011 Feb 1

PMID 21277013

Citations 196

Authors

Daiju Kitagawa

Ioannis Vakonakis

Natacha Olieric

Manuel Hilbert

Debora Keller

Vincent Olieric

Miriam Bortfeld

Michele C Erat

Isabelle Fluckiger

Pierre Gonczy

Michel O Steinmetz

Affiliations

Soon will be listed here.

Abstract

The centriole, and the related basal body, is an ancient organelle characterized by a universal 9-fold radial symmetry and is critical for generating cilia, flagella, and centrosomes. The mechanisms directing centriole formation are incompletely understood and represent a fundamental open question in biology. Here, we demonstrate that the centriolar protein SAS-6 forms rod-shaped homodimers that interact through their N-terminal domains to form oligomers. We establish that such oligomerization is essential for centriole formation in C. elegans and human cells. We further generate a structural model of the related protein Bld12p from C. reinhardtii, in which nine homodimers assemble into a ring from which nine coiled-coil rods radiate outward. Moreover, we demonstrate that recombinant Bld12p self-assembles into structures akin to the central hub of the cartwheel, which serves as a scaffold for centriole formation. Overall, our findings establish a structural basis for the universal 9-fold symmetry of centrioles.

Citing Articles

Drosophila Alms1 proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop.

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The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography.

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The homolog of Sjögren's Syndrome Nuclear Antigen 1 is required for the structural integrity of the centriole and bipolar mitotic spindle assembly.

Pfister J, Agostini L, Bournonville L, Sankaralingam P, Bell Z, Hamel V bioRxiv. 2025; .

PMID: 39803516 PMC: 11722412. DOI: 10.1101/2024.10.03.616528.

Visualizing Cartwheel Disassembly Process During Mitosis in Fixed and Live Cells by Fluorescence Microscope.

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The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.

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