Basal Body Stability and Ciliogenesis Requires the Conserved Component Poc1

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2009 Dec 17

PMID 20008567

Citations 84

Authors

Chad G Pearson

Daniel P S Osborn

Thomas H Giddings Jr

Philip L Beales

Mark Winey

Affiliations

Soon will be listed here.

Abstract

Centrioles are the foundation for centrosome and cilia formation. The biogenesis of centrioles is initiated by an assembly mechanism that first synthesizes the ninefold symmetrical cartwheel and subsequently leads to a stable cylindrical microtubule scaffold that is capable of withstanding microtubule-based forces generated by centrosomes and cilia. We report that the conserved WD40 repeat domain-containing cartwheel protein Poc1 is required for the structural maintenance of centrioles in Tetrahymena thermophila. Furthermore, human Poc1B is required for primary ciliogenesis, and in zebrafish, DrPoc1B knockdown causes ciliary defects and morphological phenotypes consistent with human ciliopathies. T. thermophila Poc1 exhibits a protein incorporation profile commonly associated with structural centriole components in which the majority of Poc1 is stably incorporated during new centriole assembly. A second dynamic population assembles throughout the cell cycle. Our experiments identify novel roles for Poc1 in centriole stability and ciliogenesis.

Citing Articles

The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography.

Li S, Fernandez J, Ruehle M, Howard-Till R, Fabritius A, Pearson C EMBO J. 2025; .

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Transcriptome analyses reveal differences in the response to temperature in Florida and Northern largemouth bass (Micropterus spp.) during early life stages.

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An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity.

Sala C, Wurtz M, Atorino E, Neuner A, Partscht P, Hoffmann T Nat Commun. 2024; 15(1):9857.

PMID: 39543170 PMC: 11564547. DOI: 10.1038/s41467-024-54247-5.

The secretory pathway in Tetrahymena is organized for efficient constitutive secretion at ciliary pockets.

Jiang Y, Kumar S, Turkewitz A iScience. 2024; 27(11):111123.

PMID: 39498308 PMC: 11532953. DOI: 10.1016/j.isci.2024.111123.

The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.

Li S, Fernandez J, Ruehle M, Howard-Till R, Fabritius A, Pearson C bioRxiv. 2024; .

PMID: 39314311 PMC: 11419100. DOI: 10.1101/2024.09.09.612100.

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