Evaluation of Cross-reactivity to Taenia Hydatigena and Echinococcus Granulosus in the Enzyme-linked Immunoelectrotransfer Blot Assay for the Diagnosis of Porcine Cysticercosis

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2019 Jan 26

PMID 30678700

Citations 11

Authors

Lucho Gomez-Puerta

Ana Vargas-Calla

Yesenia Castillo

Maria Teresa Lopez-Urbina

Pierre Dorny

Hector H Garcia

Armando E Gonzalez

Seth E ONeal

Affiliations

Soon will be listed here.

Abstract

Background: Taenia solium is an important zoonotic parasite that infects humans as definitive host (taeniasis) and pigs as intermediate host (cysticercosis). Serological diagnosis of porcine cysticercosis is limited to antigen detection using ELISA, which is known to cross-react with other Taenia species, and antibody detection using the lentil-lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB), which has not been adequately evaluated for cross-reactivity to other parasites. Field studies suggest that the GP50 diagnostic band of the LLGP EITB may cross-react to Taenia hydatigena, a common non-zoonotic parasitic infection of pigs. The objective of this study was to evaluate the specificity of the LLGP EITB assay in pigs infected experimentally with T. hydatigena and Echinococcus granulosus.

Results: Twelve three-month-old seronegative were divided into two groups; six were each given an oral challenge with a single gravid proglottid of T. hydatigena and the other six were each given an oral challenge with 50 gravid proglottids of E. granulosus. Serum samples were collected biweekly until 14 weeks when all pigs underwent a detailed necropsy. Taenia hydatigena cysticerci were found in two of six pigs from the first group. Four T. hydatigena-exposed pigs were seropositive at the GP50-band only on EITB LLGP; two of these had cysts at necropsy while no seronegative pigs had cysts. One E. granulosus-exposed pig was positive to EITB LLGP, again with reactivity only to GP50; all six pigs had hepatic echinococcosis on necropsy.

Conclusion: These results provide definitive evidence that the GP50 diagnostic band in pigs cross-reacts with T. hydatigena. Evidence of cross-reaction with E. granulosus was not conclusive.

Citing Articles

Antibody Banding Patterns on the Enzyme-Linked Immunoelectrotransfer Blot (EITB) Assay Clearly Discriminate Viable Cysticercosis in Naturally Infected Pigs.

Arroyo G, Lescano A, Gavidia C, Lopez-Urbina T, Ara-Gomez M, Gomez-Puerta L Pathogens. 2024; 13(1).

PMID: 38251323 PMC: 10820179. DOI: 10.3390/pathogens13010015.

Spatial transferability of an agent-based model to simulate Taenia solium control interventions.

Pizzitutti F, Bonnet G, Gonzales-Gustavson E, Gabriel S, Pan W, Gonzalez A Parasit Vectors. 2023; 16(1):410.

PMID: 37941062 PMC: 10634186. DOI: 10.1186/s13071-023-06003-9.

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host's Immune Response? An Integrative Overview.

Rios-Valencia D, Ambrosio J, Tirado-Mendoza R, Carrero J, Laclette J Pathogens. 2023; 12(6).

PMID: 37375530 PMC: 10302083. DOI: 10.3390/pathogens12060840.

Evaluating the Role of Corrals and Insects in the Transmission of Porcine Cysticercosis: A Cohort Study.

Gonzales-Gustavson E, Pray I, Gamboa R, Muro C, Vilchez P, Gomez-Puerta L Pathogens. 2023; 12(4).

PMID: 37111483 PMC: 10143142. DOI: 10.3390/pathogens12040597.

Non-local validated parametrization of an agent-based model of local-scale Taenia solium transmission in North-West Peru.

Pizzitutti F, Bonnet G, Gonzales-Gustavson E, Gabriel S, Pan W, Pray I PLoS One. 2022; 17(9):e0275247.

PMID: 36166462 PMC: 9514638. DOI: 10.1371/journal.pone.0275247.

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