The Complete Mitochondrial Genomes of Three Cestode Species of Taenia Infecting Animals and Humans

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2010 Oct 6

PMID 20922482

Citations 31

Authors

Guo-Hua Liu

Rui-Qing Lin

Ming-Wei Li

Wei Liu

Yi Liu

Zi-Guo Yuan

Hui-Qun Song

Guang-Hui Zhao

Kou-Xing Zhang

Xing-Quan Zhu

Affiliations

Soon will be listed here.

Abstract

Mitochondrial (mt) genome sequences provide useful markers for investigating population genetic structures, systematics and phylogenetics of organisms. Although Taenia multiceps, T. hydatigena, and T. taeniaeformis are common taeniid tapeworms of ruminants, pigs, dogs, or cats, causing significant economic losses, no published study on their mt genomes is available. The complete mt genomes of T. multiceps, T. hydatigena, and T. taeniaeformis were amplified in two overlapping fragments and then sequenced. The sizes of the entire mt genome were 13700 bp for T. multiceps, 13489 bp for T. hydatigena, and 13647 bp for T. taeniaeformis. Each of the three genomes contains 36 genes, consisting of 12 genes for proteins, 2 genes for rRNA, and 22 genes for tRNA, which are the same as the mt genomes of all other cestode species studied to date. All genes are transcribed in the same direction and have a nucleotide composition high in A and T. The contents of A+T of the complete genomes are 71.3% for T. multiceps, 70.8% for T. hydatigena, and 73.0% for T. taeniaeformis. The AT bias had a significant effect on both the codon usage pattern and amino acid composition of proteins. T. multiceps and T. hydatigena had two noncoding regions, but T. taeniaeformis had only one. Phylogenetic analyses based on concatenated amino acid sequences of 12 protein-coding genes revealed that T. multiceps, T. hydatigena, and T. taeniaeformis were more closely related to the other members of the Taenia genus, consistent with results of previous morphological and molecular studies. The present study determined the complete mt genome sequences for three Taenia species of animal and human health significance, providing useful markers for studying the systematics, population genetics, and molecular epidemiology of these cestode parasites of animals and humans.

Citing Articles

Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia.

Miljevic M, Rajicic M, Umhang G, Bajic B, Bjelic cabrilo O, Budinski I Parasit Vectors. 2023; 16(1):250.

PMID: 37491284 PMC: 10369706. DOI: 10.1186/s13071-023-05879-x.

Cestodes of the Genus Fimbriaria Froelich, 1802 (Cestoda, Cyclophyllidea, Fimbriariinae) from Ukraine, with a Key to Species Level.

Greben O, Kornyushin V, du Preez L, Melnychuk V, Yevstafieva V, Syrota Y Acta Parasitol. 2022; 68(1):145-158.

PMID: 36454399 DOI: 10.1007/s11686-022-00644-z.

Comparative Mitochondrial Genomic Analysis Robustly Supported That Cat Tapeworm (Platyhelminthes: Cestoda) Represents a Species Complex.

Wang H, Li R, Deng Y, Liu G, Fu Y Front Vet Sci. 2022; 9:931137.

PMID: 35812880 PMC: 9258744. DOI: 10.3389/fvets.2022.931137.

Heteroplasmic mitochondrial genomes of a Raillietina tapeworm in wild Pangolin.

Tuli M, Li H, Pan X, Li S, Zhai J, Wu Y Parasit Vectors. 2022; 15(1):204.

PMID: 35698206 PMC: 9195439. DOI: 10.1186/s13071-022-05301-y.

Comparative Characterization of Mitogenomes From Five Orders of Cestodes (Eucestoda: Tapeworms).

Trevisan B, Jacob Machado D, Lahr D, Marques F Front Genet. 2022; 12:788871.

PMID: 35003223 PMC: 8727539. DOI: 10.3389/fgene.2021.788871.

References

Wei S, Tang P, Zheng L, Shi M, Chen X . The complete mitochondrial genome of Evania appendigaster (Hymenoptera: Evaniidae) has low A+T content and a long intergenic spacer between atp8 and atp6. Mol Biol Rep. 2009; 37(4):1931-42. PMC: 2831182. DOI: 10.1007/s11033-009-9640-1. View

Hua J, Li M, Dong P, Xie Q, Bu W . The mitochondrial genome of Protohermes concolorus Yang et Yang 1988 (Insecta: Megaloptera: Corydalidae). Mol Biol Rep. 2008; 36(7):1757-65. DOI: 10.1007/s11033-008-9379-0. View

Nakao M, Abmed D, Yamasaki H, Ito A . Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda: Diphyllobothriidae). Parasitol Res. 2007; 101(1):233-6. DOI: 10.1007/s00436-006-0433-3. View

Wang C, Qiu J, Zhao J, Xu L, Yu W, Zhu X . Prevalence of helminthes in adult dogs in Heilongjiang Province, the People's Republic of China. Parasitol Res. 2006; 99(5):627-30. DOI: 10.1007/s00436-006-0219-7. View

Jeon H, Eom K . Taenia asiatica and Taenia saginata: genetic divergence estimated from their mitochondrial genomes. Exp Parasitol. 2006; 113(1):58-61. DOI: 10.1016/j.exppara.2005.11.018. View

Gauci C, Vural G, Oncel T, Varcasia A, Damian V, Kyngdon C . Vaccination with recombinant oncosphere antigens reduces the susceptibility of sheep to infection with Taenia multiceps. Int J Parasitol. 2007; 38(8-9):1041-50. PMC: 2706984. DOI: 10.1016/j.ijpara.2007.11.006. View

Yang Y, Rosenzvit M, Zhang L, Zhang J, McManus D . Molecular study of Echinococcus in west-central China. Parasitology. 2005; 131(Pt 4):547-55. DOI: 10.1017/S0031182005007973. View

Xiao N, Qiu J, Nakao M, Li T, Yang W, Chen X . Echinococcus shiquicus n. sp., a taeniid cestode from Tibetan fox and plateau pika in China. Int J Parasitol. 2005; 35(6):693-701. DOI: 10.1016/j.ijpara.2005.01.003. View

Boore J . Animal mitochondrial genomes. Nucleic Acids Res. 1999; 27(8):1767-80. PMC: 148383. DOI: 10.1093/nar/27.8.1767. View

10.

Mayta H, TALLEY A, Gilman R, Jimenez J, Verastegui M, Ruiz M . Differentiating Taenia solium and Taenia saginata infections by simple hematoxylin-eosin staining and PCR-restriction enzyme analysis. J Clin Microbiol. 2000; 38(1):133-7. PMC: 86038. DOI: 10.1128/JCM.38.1.133-137.2000. View

11.

Asakawa S, Himeno H, Miura K, Watanabe K . Nucleotide sequence and gene organization of the starfish Asterina pectinifera mitochondrial genome. Genetics. 1995; 140(3):1047-60. PMC: 1206660. DOI: 10.1093/genetics/140.3.1047. View

12.

Tamura K, Dudley J, Nei M, Kumar S . MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007; 24(8):1596-9. DOI: 10.1093/molbev/msm092. View

13.

Yu Z, Wei Z, Kong X, Shi W . Complete mitochondrial DNA sequence of oyster Crassostrea hongkongensis-a case of "Tandem duplication-random loss" for genome rearrangement in Crassostrea?. BMC Genomics. 2008; 9:477. PMC: 2576254. DOI: 10.1186/1471-2164-9-477. View

14.

Sissay M, Uggla A, Waller P . Prevalence and seasonal incidence of larval and adult cestode infections of sheep and goats in eastern Ethiopia. Trop Anim Health Prod. 2008; 40(6):387-94. DOI: 10.1007/s11250-007-9096-z. View

15.

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D . The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1998; 25(24):4876-82. PMC: 147148. DOI: 10.1093/nar/25.24.4876. View

16.

Benifla M, Barrelly R, Shelef I, El-On J, Cohen A, Cagnano E . Huge hemispheric intraparenchymal cyst caused by Taenia multiceps in a child. Case report. J Neurosurg. 2007; 107(6 Suppl):511-4. DOI: 10.3171/PED-07/12/511. View

17.

Okamoto M, Nakao M, Blair D, Anantaphruti M, Waikagul J, Ito A . Evidence of hybridization between Taenia saginata and Taenia asiatica. Parasitol Int. 2009; 59(1):70-4. DOI: 10.1016/j.parint.2009.10.007. View

18.

Lowe T, Eddy S . tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997; 25(5):955-64. PMC: 146525. DOI: 10.1093/nar/25.5.955. View

19.

Gasser R, Zhu X, Woods W . Genotyping Taenia tapeworms by single-strand conformation polymorphism of mitochondrial DNA. Electrophoresis. 1999; 20(14):2834-7. DOI: 10.1002/(SICI)1522-2683(19991001)20:14<2834::AID-ELPS2834>3.0.CO;2-F. View

20.

Dai R, Li Z, Li F, Liu D, Liu W, Liu G . Severe infection of adult dogs with helminths in Hunan Province, China poses significant public health concerns. Vet Parasitol. 2008; 160(3-4):348-50. DOI: 10.1016/j.vetpar.2008.11.002. View