Characterization of the 8-kilodalton Antigens of Taenia Solium Metacestodes and Evaluation of Their Use in an Enzyme-linked Immunosorbent Assay for Serodiagnosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2003 Jun 7

PMID 12791883

Citations 29

Authors

Kathy Hancock

Azra Khan

Fatima B Williams

Melinda L Yushak

Sowmya Pattabhi

John Noh

Victor C W Tsang

Affiliations

Soon will be listed here.

Abstract

The Western blot for cysticercosis, which uses lentil lectin purified glycoprotein (LLGP) antigens extracted from the metacestode of Taenia solium, has been the "gold standard" serodiagnostic assay since it was first described in 1989. We report that the diagnostic antigens at 14, 18, and 21 kDa, as well as some larger disulfide-bonded antigens, are actually all members of a very closely related family of proteins, the 8-kDa antigens. The genes for 18 unique, mature proteins have been identified. Nine of these were chemically synthesized and tested in an enzyme-linked immunosorbent assay with a battery of defined serum samples, including 32 cysticercosis-positive serum samples reactive with the 8-kDa antigens of LLGP on Western blotting, 34 serum samples from patients with other parasitic infections, and 15 normal human serum samples. One of the 8-kDa antigens, TsRS1, is 100% sensitive and 100% specific. TsRS1 will be one component of a cocktail of three to four synthetic or recombinant antigens, based on the diagnostic bands of the Western blot, which will be used for the serodiagnosis of cysticercosis.

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