Functionally Diverse Type V CRISPR-Cas Systems

Overview

Journal Science

Specialty Science

Date 2018 Dec 8

PMID 30523077

Citations 199

Authors

Winston X Yan

Pratyusha Hunnewell

Lauren E Alfonse

Jason M Carte

Elise Keston-Smith

Shanmugapriya Sothiselvam

Anthony J Garrity

Shaorong Chong

Kira S Makarova

Eugene V Koonin

David R Cheng

David A Scott

Affiliations

Soon will be listed here.

Abstract

Type V CRISPR-Cas systems are distinguished by a single RNA-guided RuvC domain-containing effector, Cas12. Although effectors of subtypes V-A (Cas12a) and V-B (Cas12b) have been studied in detail, the distinct domain architectures and diverged RuvC sequences of uncharacterized Cas12 proteins suggest unexplored functional diversity. Here, we identify and characterize Cas12c, -g, -h, and -i. Cas12c, -h, and -i demonstrate RNA-guided double-stranded DNA (dsDNA) interference activity. Cas12i exhibits markedly different efficiencies of CRISPR RNA spacer complementary and noncomplementary strand cleavage resulting in predominant dsDNA nicking. Cas12g is an RNA-guided ribonuclease (RNase) with collateral RNase and single-strand DNase activities. Our study reveals the functional diversity emerging along different routes of type V CRISPR-Cas evolution and expands the CRISPR toolbox.

Citing Articles

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