The Biological Response to Laser-aided Direct Metal-coated Titanium Alloy (Ti6Al4V)

Overview

Journal Bone Joint Res

Date 2018 Jun 21

PMID 29922456

Citations 1

Authors

T Shin

D Lim

Y S Kim

S C Kim

W L Jo

Y W Lim

Affiliations

Soon will be listed here.

Abstract

Objectives: Laser-engineered net shaping (LENS) of coated surfaces can overcome the limitations of conventional coating technologies. We compared the biological response with a titanium plasma spray (TPS)-coated titanium alloy (Ti6Al4V) surface with that of a Ti6Al4V surface coated with titanium using direct metal fabrication (DMF) with 3D printing technologies.

Methods: The ability of human osteoblasts to adhere to TPS-coated Ti6Al4V was compared with DMF-coating. Scanning electron microscopy (SEM) was used to assess the structure and morphology of the surfaces. Biological and morphological responses to human osteoblast cell lines were then examined by measuring cell proliferation, alkaline phosphatase activity, actin filaments, and RUNX2 gene expression.

Results: Morphological assessment of the cells after six hours of incubation using SEM showed that the TPS- and DMF-coated surfaces were largely covered with lamellipodia from the osteoblasts. Cell adhesion appeared similar in both groups. The differences in the rates of cell proliferation and alkaline phosphatase activities were not statistically significant.

Conclusions: The DMF coating applied using metal 3D printing is similar to the TPS coating, which is the most common coating process used for bone ingrowth. The DMF method provided an acceptable surface structure and a viable biological surface. Moreover, this method is automatable and less complex than plasma spraying.: T. Shin, D. Lim, Y. S. Kim, S. C. Kim, W. L. Jo, Y. W. Lim. The biological response to laser-aided direct metal-coated Titanium alloy (Ti6Al4V). 2018;7:357-361. DOI: 10.1302/2046-3758.75.BJR-2017-0222.R1.

Citing Articles

Comparative study of the osseointegration of 3D-printed and plasma-coated titanium implants.

Bondarenko S, Filipenko V, Ashukina N, Maltseva V, Ivanov G, Lazarenko I World J Orthop. 2023; 14(9):682-689.

PMID: 37744721 PMC: 10514715. DOI: 10.5312/wjo.v14.i9.682.

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