Characterization of a Q Subregion Gene in the Murine Major Histocompatibility Complex

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Mar 1

PMID 2983348

Citations 19

Authors

L Flaherty

K DiBiase

M A Lynes

J G Seidman

O Weinberger

E M Rinchik

Affiliations

Soon will be listed here.

Abstract

We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.

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