Expression of the Q8/9d Gene by T Cells of the Mouse

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1989 Jan 1

PMID 2789189

Citations 6

Authors

A Matsuura

R Schloss

F W Shen

J S Tung

S W Hunt 3rd

D A Fisher

L E HOOD

E A Boyse

Affiliations

Soon will be listed here.

Abstract

The Q genes, specifying Qa antigens and situated in the extended part of the major histocompatibility complex (MHC) of the mouse, comprise a subgroup of MHC class I genes whose significance and function are still largely unknown. In screening a cDNA library made from the BALB/c inducer T-cell line Cl.Ly1-T1, we isolated 11 clones representing Q8/9, but none representing Q6 or Q7. Confirmatory evidence is given that the Q8/9 gene originated from fusion of the 5' region of the Q8 gene with the 3' region of the Q9 gene at a recombination site or hot spot in the vicinity of intron 4. Contrary to previous impressions that Q8/9 is an inert pseudogene, we find that the Q8/9 gene can be functional and encode a Qa-2, 3 antigen. One variety of the 11 Q8/9 clones isolated lacked exon 5, which encodes the transmembrane domain of class I glycoproteins, and thus may account for secretion of a soluble form of Qa-2, 3 antigen thought to be released by activated T cells.

Citing Articles

A physical map of the Q region of B1O.P.

Litaker W, Frelinger J Mamm Genome. 1996; 7(3):200-5.

PMID: 8833240 DOI: 10.1007/s003359900054.

A new polymorphic and multicopy MHC gene family related to nonmammalian class I.

Leelayuwat C, Townend D, Degli-Esposti M, Abraham L, Dawkins R Immunogenetics. 1994; 40(5):339-51.

PMID: 7927538 DOI: 10.1007/BF01246675.

New nucleotide sequence data on the EMBL File Server.

Nucleic Acids Res. 1990; 18(3):695-700.

PMID: 2308868 PMC: 333521. DOI: 10.1093/nar/18.3.695.

Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen.

Ulker N, Lewis K, HOOD L, Stroynowski I EMBO J. 1990; 9(12):3839-47.

PMID: 2249652 PMC: 552151. DOI: 10.1002/j.1460-2075.1990.tb07602.x.

MHC gene Q8/9d of the BALB/cJ mouse strain cannot encode a Qa-2,3 class I antigen.

Nakayama K, TOKITO S, Pannetier C, Nakauchi H, Gachelin G Immunogenetics. 1991; 33(4):225-34.

PMID: 2026459 DOI: 10.1007/BF00230499.

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