Expression and Regulation of Q8b in a Transfected Cell Line

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1991 Jan 1

PMID 1654299

Authors

J B Waters

L Flaherty

Affiliations

Soon will be listed here.

Abstract

RNase protection experiments showed that Q8b was actively transcribed in a stably transfected cell line. Moreover, Q8b responded to interferon-gamma (IFN-gamma) treatment with increased levels of mRNA expression. Thus Q8b demonstrates a regulatory response to IFN-gamma characteristic of many other class I genes. Cell surface expression of a Q8b product could also be detected by flow cytometric analysis with the Qa-2-specific monoclonal antibody D3.262. The expression of the Q8b cell surface product increased only slightly after cells were treated with IFN-gamma. The Q8b cell surface product was not sensitive to cleavage by phosphatidylinositol-phospholipase C. These results suggest that the Q8b product, unlike the predominant forms of Qa-2-bearing molecules, is not anchored via phosphatidylinositol to the cell membrane. These results also suggest that Q8b has the potential to contribute to the Qa-2 phenotype in vivo.

References

Low M, Kincade P . Phosphatidylinositol is the membrane-anchoring domain of the Thy-1 glycoprotein. Nature. 1985; 318(6041):62-4. DOI: 10.1038/318062a0. View

Marshak-Rothstein A, Fink P, Gridley T, Raulet D, Bevan M, Gefter M . Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979; 122(6):2491-7. View

Shirayoshi Y, Burke P, Appella E, Ozato K . Interferon-induced transcription of a major histocompatibility class I gene accompanies binding of inducible nuclear factors to the interferon consensus sequence. Proc Natl Acad Sci U S A. 1988; 85(16):5884-8. PMC: 281869. DOI: 10.1073/pnas.85.16.5884. View

Houlden B, Matis L, Cron R, Widacki S, Brown G, Pampeno C . A TCR gamma delta cell recognizing a novel TL-encoded gene product. Cold Spring Harb Symp Quant Biol. 1989; 54 Pt 1:45-55. DOI: 10.1101/sqb.1989.054.01.006. View

Mellor A, Antoniou J, Robinson P . Structure and expression of genes encoding murine Qa-2 class I antigens. Proc Natl Acad Sci U S A. 1985; 82(17):5920-4. PMC: 390665. DOI: 10.1073/pnas.82.17.5920. View

Stroynowski I, Soloski M, Low M, Hood L . A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen: anchoring of the product by a phospholipid tail. Cell. 1987; 50(5):759-68. DOI: 10.1016/0092-8674(87)90334-5. View

Soloski M, Lattimore A, Hereld D, Krakow J, Low M, Einhorn G . Further characterization of the membrane anchor found on the tissue-specific class I molecule Qa2. J Immunol. 1988; 140(11):3858-66. View

Townsend A, Ohlen C, Bastin J, Ljunggren H, Foster L, Karre K . Association of class I major histocompatibility heavy and light chains induced by viral peptides. Nature. 1989; 340(6233):443-8. DOI: 10.1038/340443a0. View

Oi V, Jones P, Goding J, Herzenberg L . Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978; 81:115-20. DOI: 10.1007/978-3-642-67448-8_18. View

10.

Mann D, Forman J . Recognition by cytotoxic T lymphocytes of Qa-2 antigens. Sensitivity of Qa-2 molecules to phosphatidylinositol-specific phospholipase C. J Immunol. 1988; 141(6):1813-8. View

11.

Stroynowski I, Forman J, Goodenow R, Schiffer S, McMillan M, Sharrow S . Expression and T cell recognition of hybrid antigens with amino-terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens. J Exp Med. 1985; 161(5):935-52. PMC: 2187603. DOI: 10.1084/jem.161.5.935. View

12.

Sherman D, Waneck G, Flavell R . Qa-2 antigen encoded by Q7b is biochemically indistinguishable from Qa-2 expressed on the surface of C57BL/10 mouse spleen cells. J Immunol. 1988; 140(1):138-42. View

13.

Widacki S, Cook R . NH2-terminal amino acid sequence analysis of Qa-2 alloantigens. Immunogenetics. 1989; 29(3):206-9. DOI: 10.1007/BF00373647. View

14.

Soloski M, Vernachio J, Einhorn G, Lattimore A . Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells. Proc Natl Acad Sci U S A. 1986; 83(9):2949-53. PMC: 323424. DOI: 10.1073/pnas.83.9.2949. View

15.

Lie W, Myers N, Connolly J, Gorka J, Lee D, Hansen T . The specific binding of peptide ligand to Ld class I major histocompatibility complex molecules determines their antigenic structure. J Exp Med. 1991; 173(2):449-59. PMC: 2118782. DOI: 10.1084/jem.173.2.449. View

16.

Elliott E, Karl M, Flaherty L . Novel mRNA species from a Q-subregion class-I gene of the murine major histocompatibility complex. Gene. 1987; 61(2):189-98. DOI: 10.1016/0378-1119(87)90113-2. View

17.

Soloski M, Hood L, Stroynowski I . Qa-region class I gene expression: identification of a second class I gene, Q9, encoding a Qa-2 polypeptide. Proc Natl Acad Sci U S A. 1988; 85(9):3100-4. PMC: 280151. DOI: 10.1073/pnas.85.9.3100. View

18.

Tonegawa S, Berns A, Bonneville M, Farr A, Ishida I, Ito K . Diversity, development, ligands, and probable functions of gamma delta T cells. Cold Spring Harb Symp Quant Biol. 1989; 54 Pt 1:31-44. DOI: 10.1101/sqb.1989.054.01.005. View

19.

Vidovic D, Roglic M, McKune K, Guerder S, MacKay C, Dembic Z . Qa-1 restricted recognition of foreign antigen by a gamma delta T-cell hybridoma. Nature. 1989; 340(6235):646-50. DOI: 10.1038/340646a0. View

20.

Korber B, Mermod N, Hood L, Stroynowski I . Regulation of gene expression by interferons: control of H-2 promoter responses. Science. 1988; 239(4845):1302-6. DOI: 10.1126/science.3125612. View