A Physical Map of the Q Region of B1O.P

Overview

Journal Mamm Genome

Specialty Genetics

Date 1996 Mar 1

PMID 8833240

Citations 1

Authors

W Litaker

J A Frelinger

Affiliations

Soon will be listed here.

Abstract

The murine class I MHC Q region is part of a large complex multigene family whose members have various peptide binding functions. The structure of the Q region is complex, varying extensively in the b, d, k, and q haplotypes so far examined. To better understand the structural heterogeneity, we examined the Q region of B 10.P, a strain whose immunological characteristics are distinct from other haplotypes. A total of 89 cosmids were isolated from genomic DNA. The B 10.P Q region was found to contain seven genes in a 190-kb cluster linked to DP and two additional Q genes in a separate 55-kb cluster. The gene arrangement in this haplotype was unique and did not correspond to any other haplotype; this underscores the complexity of chromosomal structure in this region. In addition to the Q region clusters, Tla region was tentatively aligned in five clusters spanning approximately 300 kb. One 37-kb M region cosmid was also identified.

Citing Articles

Murine MHC class Ib gene, H2-M2, encodes a conserved surface-expressed glycoprotein.

Moore Y, Lambracht-Washington D, Tabaczewski P, Lindahl K Immunogenetics. 2004; 56(1):1-11.

PMID: 15045471 DOI: 10.1007/s00251-004-0661-6.

References

Parham P, Lawlor D, Lomen C, Ennis P . Diversity and diversification of HLA-A,B,C alleles. J Immunol. 1989; 142(11):3937-50. View

Fischer Lindahl K, Hermel E, Loveland B, Wang C . Maternally transmitted antigen of mice: a model transplantation antigen. Annu Rev Immunol. 1991; 9:351-72. DOI: 10.1146/annurev.iy.09.040191.002031. View

Weiss E . Molecular biology of the mouse Q region. Immunol Res. 1987; 6(3):179-91. DOI: 10.1007/BF02918090. View

Steinmetz M, Frelinger J, Fisher D, Hunkapiller T, Pereira D, Weissman S . Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981; 24(1):125-34. DOI: 10.1016/0092-8674(81)90508-0. View

Nakayama K, TOKITO S, Jaulin C, Delarbre C, Kourilsky P, Nakauchi H . Comparative structure of two duplicated T1a class I genes (T10c and 37) of the murine H-2d MHC. Implications on the evolution of the T1a region. J Immunol. 1990; 144(6):2400-8. View

Mellor A, Antoniou J, Robinson P . Structure and expression of genes encoding murine Qa-2 class I antigens. Proc Natl Acad Sci U S A. 1985; 82(17):5920-4. PMC: 390665. DOI: 10.1073/pnas.82.17.5920. View

Winoto A, Steinmetz M, Hood L . Genetic mapping in the major histocompatibility complex by restriction enzyme site polymorphisms: most mouse class I genes map to the Tla complex. Proc Natl Acad Sci U S A. 1983; 80(11):3425-9. PMC: 394056. DOI: 10.1073/pnas.80.11.3425. View

Stroynowski I, Soloski M, Low M, Hood L . A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen: anchoring of the product by a phospholipid tail. Cell. 1987; 50(5):759-68. DOI: 10.1016/0092-8674(87)90334-5. View

Brorson K, Richards S, Hunt 3rd S, Cheroutre H, Lindahl K, Hood L . Analysis of a new class I gene mapping to the Hmt region of the mouse. Immunogenetics. 1989; 30(4):273-83. DOI: 10.1007/BF02421331. View

10.

Ronne H, Widmark E, Rask L, Peterson P . Intron sequences reveal evolutionary relationships among major histocompatibility complex class I genes. Proc Natl Acad Sci U S A. 1985; 82(17):5860-4. PMC: 390653. DOI: 10.1073/pnas.82.17.5860. View

11.

Palmer M, Frelinger J . Widespread transcription of a Qa region gene in adult mice. J Exp Med. 1987; 166(1):95-108. PMC: 2188633. DOI: 10.1084/jem.166.1.95. View

12.

Mellor A, Weiss E, Kress M, Jay G, Flavell R . A nonpolymorphic class I gene in the murine major histocompatibility complex. Cell. 1984; 36(1):139-44. DOI: 10.1016/0092-8674(84)90082-5. View

13.

Weiss E, Mellor A, Golden L, Fahrner K, Simpson E, Hurst J . The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events. Nature. 1983; 301(5902):671-4. DOI: 10.1038/301671a0. View

14.

Singer D, Hare J, Golding H, Flaherty L, Rudikoff S . Characterization of a new subfamily of class I genes in the H-2 complex of the mouse. Immunogenetics. 1988; 28(1):13-21. DOI: 10.1007/BF00372524. View

15.

Atchley W, FITCH W . Gene trees and the origins of inbred strains of mice. Science. 1991; 254(5031):554-8. DOI: 10.1126/science.1948030. View

16.

Macchi M, Woodward J, Griffin J, Hood L, Frelinger J . Cloning and identification of the H-2Dp gene. Immunogenetics. 1984; 19(3):195-204. DOI: 10.1007/BF00364763. View

17.

Hasenkrug K, Stimpfling J . The antigenic properties of twenty-four new H-2 congenic recombinant mouse strains. Immunogenetics. 1986; 24(6):423-7. DOI: 10.1007/BF00377962. View

18.

Ito K, Van Kaer L, Bonneville M, Hsu S, Murphy D, Tonegawa S . Recognition of the product of a novel MHC TL region gene (27b) by a mouse gamma delta T cell receptor. Cell. 1990; 62(3):549-61. DOI: 10.1016/0092-8674(90)90019-b. View

19.

Pease L, Horton R, Pullen J, Cai Z . Structure and diversity of class I antigen presenting molecules in the mouse. Crit Rev Immunol. 1991; 11(1):1-32. View

20.

Stephan D, Sun H, Lindahl K, Meyer E, Hammerling G, Hood L . Organization and evolution of D region class I genes in the mouse major histocompatibility complex. J Exp Med. 1986; 163(5):1227-44. PMC: 2188107. DOI: 10.1084/jem.163.5.1227. View