Comparative Cell Cycle Transcriptomics Reveals Synchronization of Developmental Transcription Factor Networks in Cancer Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Dec 12

PMID 29228002

Citations 15

Authors

Johan Bostrom

Zuzana Sramkova

Alena Salasova

Helena Johard

Diana Mahdessian

Radek Fedr

Carolyn Marks

Jirina Medalova

Karel Soucek

Emma Lundberg

Sten Linnarsson

Vitezslav Bryja

Petra Sekyrova

Mikael Altun

Michael Andang

Affiliations

Soon will be listed here.

Abstract

The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.

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