STRT-seq-2i: Dual-index 5' Single Cell and Nucleus RNA-seq on an Addressable Microwell Array

Overview

Journal Sci Rep

Specialty Science

Date 2017 Nov 29

PMID 29180631

Citations 38

Authors

Hannah Hochgerner

Peter Lonnerberg

Rebecca Hodge

Jaromir Mikes

Abeer Heskol

Hermann Hubschle

Philip Lin

Simone Picelli

Gioele La Manno

Michael Ratz

Jude Dunne

Syed Husain

Ed Lein

Maithreyan Srinivasan

Amit Zeisel

Sten Linnarsson

Affiliations

Soon will be listed here.

Abstract

Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilution or FACS, with imaging and high throughput at competitive cost. We applied the platform to fresh single mouse cortical cells and to frozen post-mortem human cortical nuclei, matching the performance of a previous lower-throughput platform while retaining a high degree of flexibility, potentially also for other high-throughput applications.

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