Addressing Preferred Specimen Orientation in Single-particle Cryo-EM Through Tilting

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2017 Jul 4

PMID 28671674

Citations 505

Authors

Yong Zi Tan

Philip R Baldwin

Joseph H Davis

James R Williamson

Clinton S Potter

Bridget Carragher

Dmitry Lyumkis

Affiliations

Soon will be listed here.

Abstract

We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations.

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