Interaction of Complexes I, III, and IV Within the Bovine Respirasome by Single Particle Cryoelectron Tomography

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2011 Aug 31

PMID 21876144

Citations 84

Authors

Natalya V Dudkina

Mikhail Kudryashev

Henning Stahlberg

Egbert J Boekema

Affiliations

Soon will be listed here.

Abstract

The respirasome is a multisubunit supercomplex of the respiratory chain in mitochondria. Here we report the 3D reconstruction of the bovine heart respirasome, composed of dimeric complex III and single copies of complex I and IV, at about 2.2-nm resolution, determined by cryoelectron tomography and subvolume averaging. Fitting of X-ray structures of single complexes I, III(2), and IV with high fidelity allows interpretation of the model at the level of secondary structures and shows how the individual complexes interact within the respirasome. Surprisingly, the distance between cytochrome c binding sites of complexes III(2) and IV is about 10 nm. Modeling indicates a loose interaction between the three complexes and provides evidence that lipids are gluing them at the interfaces.

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