A Synthetic Peptide Corresponding to the Cleavage Region of VP3 from Rotavirus SA11 Induces Neutralizing Antibodies

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Nov 1

PMID 2845138

Citations 10

Authors

H J Streckert

H Brussow

H Werchau

Affiliations

Soon will be listed here.

Abstract

Antibodies were elicited in rabbits by immunization with the synthetic tetradecapeptide Gln-Asn-Thr-Arg-Asn-Ile-Val-Pro-Val-Ser-Ile-Val-Ser-Arg, corresponding to amino acids 228 to 241 of SA11-VP3. Protein specificity of the antipeptide serum is demonstrated. The antipeptide serum revealed neutralizing activity directed against SA11 in a neutralization assay. Human rotavirus strains Wa, S2, and Hochi and bovine strains NCDV and UK were not neutralized, demonstrating the strain-specific neutralizing activity of the raised antipeptide serum. Upon immune electron microscopy, aggregation of SA11 particles was observed.

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