Operational Overlapping of Cross-reactive and Serotype-specific Neutralization Epitopes on VP7 of Human Rotavirus Serotype 3

Overview

Journal Arch Virol

Specialty Microbiology

Date 1991 Jan 1

PMID 1706592

Citations 2

Authors

N Kobayashi

K Taniguchi

S URASAWA

Affiliations

Soon will be listed here.

Abstract

VP7-specific neutralizing monoclonal antibodies (N-MAbs) to serotype 3 human rotavirus were produced to analyze serotype 3-specific and cross-reactive neutralization epitopes on VP7. On the basis of the reactivity patterns in neutralization tests with various human and animal strains, a total of 10 N-MAbs could be classified into four groups; five antibodies specific to serotype 3 were divided into two groups, and five antibodies consisted of two groups which are cross-reactive with strain 69 M (serotype 8) or strain WI61 (serotype 9). Seven N-MAbs showed the same reactivity patterns to the virus strains in both neutralization tests and enzyme-linked immunosorbent assay (ELISA), while three N-MAbs specific to serotype 3 in neutralization showed a cross-reactivity with the serotype 8 strain in ELISA. Neutralization-resistant mutants of serotype 3 strains P and YO were selected by the N-MAbs. Cross-neutralization tests between the mutants and the MAbs indicated the presence of two serotype-specific (S1 and S2) and three cross-reactive (C1, C2, and C3) epitope groups. S1, S2, and C3 epitope groups overlapped operationally each other, and the S1 epitope group had an overlapping with the C1 epitope group. However, C2 epitope group identified by the MAbs which neutralized serotypes 3 and 9, had no operational overlapping with any other epitope groups.

Citing Articles

Characterization of the neutralizing epitopes of VP7 of the Gottfried strain of porcine rotavirus.

Kang S, Benfield D, Gorziglia M, Saif L J Clin Microbiol. 1993; 31(9):2291-7.

PMID: 7691871 PMC: 265749. DOI: 10.1128/jcm.31.9.2291-2297.1993.

Preparation and characterization of a neutralizing monoclonal antibody directed to VP4 of rotavirus strain K8 which has unique VP4 neutralization epitopes.

Kobayashi N, Taniguchi K, Urasawa T, URASAWA S Arch Virol. 1991; 121(1-4):153-62.

PMID: 1722090 DOI: 10.1007/BF01316751.

References

URASAWA S, Urasawa T, Taniguchi K, Chiba S . Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Arch Virol. 1984; 81(1-2):1-12. DOI: 10.1007/BF01309292. View

Taniguchi K, Maloy W, Nishikawa K, Green K, Hoshino Y, URASAWA S . Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. J Virol. 1988; 62(7):2421-6. PMC: 253400. DOI: 10.1128/JVI.62.7.2421-2426.1988. View

Green K, Hoshino Y, IKEGAMI N . Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology. 1989; 168(2):429-33. DOI: 10.1016/0042-6822(89)90289-4. View

Taniguchi K, Urasawa T, Morita Y, Greenberg H, URASAWA S . Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987; 155(6):1159-66. DOI: 10.1093/infdis/155.6.1159. View

Green K, Midthun K, Gorziglia M, Hoshino Y, Kapikian A, Chanock R . Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987; 161(1):153-9. DOI: 10.1016/0042-6822(87)90181-4. View

Mackow E, Barnett J, Chan H, Greenberg H . The rhesus rotavirus outer capsid protein VP4 functions as a hemagglutinin and is antigenically conserved when expressed by a baculovirus recombinant. J Virol. 1989; 63(4):1661-8. PMC: 248416. DOI: 10.1128/JVI.63.4.1661-1668.1989. View

Estes M, Palmer E, Obijeski J . Rotaviruses: a review. Curr Top Microbiol Immunol. 1983; 105:123-84. DOI: 10.1007/978-3-642-69159-1_3. View

Morita Y, Taniguchi K, Urasawa T, URASAWA S . Analysis of serotype-specific neutralization epitopes on VP7 of human rotavirus by the use of neutralizing monoclonal antibodies and antigenic variants. J Gen Virol. 1988; 69 ( Pt 2):451-8. DOI: 10.1099/0022-1317-69-2-451. View

Midthun K, Greenberg H, Hoshino Y, Kapikian A, Wyatt R, Chanock R . Reassortant rotaviruses as potential live rotavirus vaccine candidates. J Virol. 1985; 53(3):949-54. PMC: 254731. DOI: 10.1128/JVI.53.3.949-954.1985. View

10.

Andrew M, Boyle D, Coupar B, Whitfeld P, Both G, Bellamy A . Vaccinia virus recombinants expressing the SA11 rotavirus VP7 glycoprotein gene induce serotype-specific neutralizing antibodies. J Virol. 1987; 61(4):1054-60. PMC: 254062. DOI: 10.1128/JVI.61.4.1054-1060.1987. View

11.

Arias C, Garcia G, Lopez S . Priming for rotavirus neutralizing antibodies by a VP4 protein-derived synthetic peptide. J Virol. 1989; 63(12):5393-8. PMC: 251206. DOI: 10.1128/JVI.63.12.5393-5398.1989. View

12.

Mackow E, Shaw R, Matsui S, Vo P, Dang M, Greenberg H . The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proc Natl Acad Sci U S A. 1988; 85(3):645-9. PMC: 279611. DOI: 10.1073/pnas.85.3.645. View

13.

Lazdins I, Sonza S, Dyall-Smith M, Coulson B, HOLMES I . Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA11 rotavirus. J Virol. 1985; 56(1):317-9. PMC: 252539. DOI: 10.1128/JVI.56.1.317-319.1985. View

14.

Glass R, Keith J, Nakagomi O, Nakagomi T, Askaa J, Kapikian A . Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology. 1985; 141(2):292-8. DOI: 10.1016/0042-6822(85)90260-0. View

15.

Taniguchi K, Hoshino Y, Nishikawa K, Green K, Maloy W, Morita Y . Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol. 1988; 62(6):1870-4. PMC: 253268. DOI: 10.1128/JVI.62.6.1870-1874.1988. View

16.

Gerna G, Sarasini A, di Matteo A, Passarani N, Gagliardi V, Milanesi G . The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes. J Gen Virol. 1988; 69 ( Pt 4):937-44. DOI: 10.1099/0022-1317-69-4-937. View

17.

Clark H, Hoshino Y, Bell L, Groff J, Hess G, Bachman P . Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987; 25(9):1757-62. PMC: 269322. DOI: 10.1128/jcm.25.9.1757-1762.1987. View

18.

URASAWA S, Urasawa T, Taniguchi K, Morita Y, Sakurada N, Saeki Y . Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens. Microbiol Immunol. 1988; 32(7):699-708. DOI: 10.1111/j.1348-0421.1988.tb01431.x. View

19.

Ward R, Knowlton D, Schiff G, Hoshino Y, Greenberg H . Relative concentrations of serum neutralizing antibody to VP3 and VP7 proteins in adults infected with a human rotavirus. J Virol. 1988; 62(5):1543-9. PMC: 253180. DOI: 10.1128/JVI.62.5.1543-1549.1988. View

20.

Matsuno S, Hasegawa A, Mukoyama A, Inouye S . A candidate for a new serotype of human rotavirus. J Virol. 1985; 54(2):623-4. PMC: 254837. DOI: 10.1128/JVI.54.2.623-624.1985. View