Recognition of SV40-VP2 in the Infected Cell by Antipeptide Antibodies

Overview

Journal Arch Virol

Specialty Microbiology

Date 1992 Jan 1

PMID 1314053

Citations 1

Authors

H J Streckert

H J Sommerfeld

K Morgenroth

H Werchau

Affiliations

Soon will be listed here.

Abstract

Antipeptide antibodies were elicited against two synthetic peptides corresponding to amino acids 47-55 and 98-103 of the structural protein VP 2 of SV 40. The induced antibodies proved to be VP 2-specific in an immunoblot. In immunofluorescence these antibodies showed a discrete nuclear and perinuclear staining pattern. In immune electron microscopy studies the induced antibodies did not bind to major virions suggesting that VP 2 is not present at the surface of SV 40 particles.

Citing Articles

Antibodies reacting with JCPyV_VP2 _167-15mer as a novel serological marker for JC polyomavirus infection.

Lagatie O, Loy T, Tritsmans L, Stuyver L Virol J. 2014; 11:174.

PMID: 25273879 PMC: 4194363. DOI: 10.1186/1743-422X-11-174.

References

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Tegtmeyer P, ROBB J, Widmer C, Ozer H . Altered protein metabolism in infection by the late tsB11 mutant of simian virus 40. J Virol. 1974; 14(4):997-1007. PMC: 355607. DOI: 10.1128/JVI.14.4.997-1007.1974. View

Streuli C, Griffin B . Myristic acid is coupled to a structural protein of polyoma virus and SV40. Nature. 1987; 326(6113):619-22. DOI: 10.1038/326619a0. View

Gharakhanian E, Takahashi J, Kasamatsu H . The carboxyl 35 amino acids of SV40 Vp3 are essential for its nuclear accumulation. Virology. 1987; 157(2):440-8. DOI: 10.1016/0042-6822(87)90286-8. View

Celma M, Dhar R, Pan J, Weissman S . Comparison of the nucleotide sequence of the messenger RNA for the major structural protein of SV40 with the DNA sequence encoding the amino acids of the protein. Nucleic Acids Res. 1977; 4(8):2549-50. PMC: 342591. View

Kasamatsu H, Nehorayan A . Vp1 affects intracellular localization of Vp3 polypeptide during simian virus 40 infection. Proc Natl Acad Sci U S A. 1979; 76(6):2808-12. PMC: 383698. DOI: 10.1073/pnas.76.6.2808. View

Sedman S, Mertz J . Mechanisms of synthesis of virion proteins from the functionally bigenic late mRNAs of simian virus 40. J Virol. 1988; 62(3):954-61. PMC: 253654. DOI: 10.1128/JVI.62.3.954-961.1988. View

Walter G, Roblin R, DULBECCO R . Protein synthesis in Simian virus 40-infected monkey cells. Proc Natl Acad Sci U S A. 1972; 69(4):921-4. PMC: 426595. DOI: 10.1073/pnas.69.4.921. View

Wychowski C, Benichou D, Girard M . The intranuclear location of simian virus 40 polypeptides VP2 and VP3 depends on a specific amino acid sequence. J Virol. 1987; 61(12):3862-9. PMC: 256004. DOI: 10.1128/JVI.61.12.3862-3869.1987. View

10.

Contreras R, Rogiers R, Van de Voorde A, Fiers W . Overlapping of the VP2-VP3 gene and the VP1 gene in the SV40 genome. Cell. 1977; 12(2):529-38. DOI: 10.1016/0092-8674(77)90129-5. View

11.

Good P, Welch R, Barkan A, Somasekhar M, Mertz J . Both VP2 and VP3 are synthesized from each of the alternative spliced late 19S RNA species of simian virus 40. J Virol. 1988; 62(3):944-53. PMC: 253653. DOI: 10.1128/JVI.62.3.944-953.1988. View

12.

SWEET B, HILLEMAN M . The vacuolating virus, S.V. 40. Proc Soc Exp Biol Med. 1960; 105:420-7. DOI: 10.3181/00379727-105-26128. View

13.

Kasamatsu H . Isolation of a monoclonal antibody viral polypeptide VP2 of simian virus 40. J Virol. 1982; 44(1):413-8. PMC: 256276. DOI: 10.1128/JVI.44.1.413-418.1982. View

14.

Streckert H, Werchau H . Three amino acids of a heptapeptide-induced antibody are responsible for recognition of SV40 structural proteins. Immunobiology. 1988; 177(2):149-57. DOI: 10.1016/s0171-2985(88)80035-4. View

15.

Salunke D, Caspar D, Garcea R . Self-assembly of purified polyomavirus capsid protein VP1. Cell. 1986; 46(6):895-904. DOI: 10.1016/0092-8674(86)90071-1. View

16.

Streckert H, Brussow H, Werchau H . A synthetic peptide corresponding to the cleavage region of VP3 from rotavirus SA11 induces neutralizing antibodies. J Virol. 1988; 62(11):4265-9. PMC: 253860. DOI: 10.1128/JVI.62.11.4265-4269.1988. View

17.

Streckert H, Brussow H, Sure K, Werchau H . Antipeptide antibodies directed against the carboxy-terminal region of SV40 structural proteins VP2 and VP3. J Cell Biochem. 1986; 31(4):277-87. DOI: 10.1002/jcb.240310405. View

18.

GRANBOULAN N, Tournier P, Wicker R, Bernhard W . An electron microscope study of the development of SV40 virus. J Cell Biol. 1963; 17:423-41. PMC: 2106205. DOI: 10.1083/jcb.17.2.423. View

19.

Krauzewicz N, Streuli C, Stuart-Smith N, Jones M, Wallace S, Griffin B . Myristylated polyomavirus VP2: role in the life cycle of the virus. J Virol. 1990; 64(9):4414-20. PMC: 247910. DOI: 10.1128/JVI.64.9.4414-4420.1990. View

20.

Reddy V, Ghosh P, Lebowitz P, Weissman S . Gaps and duplicated sequences in the leaders of SV40 16S RNA. Nucleic Acids Res. 1978; 5(11):4195-213. PMC: 342743. DOI: 10.1093/nar/5.11.4195. View