Neutralization Epitopes on Rotavirus SA11 4fM Outer Capsid Proteins

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Sep 1

PMID 1696640

Citations 18

Authors

M Gorziglia

G Larralde

R L Ward

Affiliations

Soon will be listed here.

Abstract

The VP7 and VP4 genes of seven antigenic mutants of simian rotavirus SA11 4fM (serotype 3) selected after 39 passages in the presence of SA11 4fM hyperimmune antiserum, were sequenced. Nucleotide sequence analysis indicated the following. (i) Twice as many amino acid substitutions occurred in the VP7 protein than in VP4, which has a molecular weight twice that of VP7. (ii) Most amino acid changes that occurred clustered in six variable regions of VP7 and in two variable regions of VP4; these variable regions may represent immunodominant epitopes. (iii) Most amino acid substitutions that occurred in VP7 and VP4 of these mutants were also observed in antigenic mutants selected with neutralizing monoclonal antibodies (NMAbs); however, some amino acid substitutions occurred that were not selected for NMAbs. (iv) On VP7, some of the neutralization epitopes appeared to be interrelated because amino acid substitution in one site affected binding of specific NMAbs to other sites, while other neutralization epitopes on VP7 appeared to be independent, in that amino acid substitution in one site did not affect the binding of NMAbs to another distant site.

Citing Articles

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Kanai Y, Kawagishi T, Nouda R, Onishi M, Pannacha P, Nurdin J J Virol. 2018; 93(4).

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Multiple Introductions and Antigenic Mismatch with Vaccines May Contribute to Increased Predominance of G12P[8] Rotaviruses in the United States.

Ogden K, Tan Y, Akopov A, Stewart L, McHenry R, Fonnesbeck C J Virol. 2018; 93(1).

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Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record.

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Characterization of a G11,P[4] strain of human rotavirus isolated in South Korea.

Hong S, Lee S, Lee S, Kang J, Lee J, Kim J J Clin Microbiol. 2007; 45(11):3759-61.

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Effect of rotavirus strain on the murine model of biliary atresia.

Allen S, Jafri M, Donnelly B, McNeal M, Witte D, Bezerra J J Virol. 2006; 81(4):1671-9.

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